IQ 1是一种Wnt/β-catenin/CBP信号维持剂。IQ 1 通过增强β-catenin/CBP介导的转录并防止转换到β-catenin/p300介导的转录来维持 Wnt/β-catenin 驱动的小鼠胚胎干细胞 (ESCs) 长期扩增并防止自发分化。IQ-1 通过与PR72/130的相互作用调节Wnt信号传导。IQ 1 可用于 ESCs 扩增的研究。
| 生物活性 | IQ 1 is aWnt/β-catenin/CBPsignalling sustainer. IQ 1 maintains long-term expansion ofWnt/β-catenin-driven mouse embryonic stem cells (ESCs) and prevents spontaneous differentiation by enhancingβ-catenin/CBP-mediated transcription and preventing conversion toβ-catenin/p300-mediated transcription. IQ-1 regulatesWntsignalling by interacting withPR72/130. IQ 1 can be used in study of ESCs expansion[1]. |
体外研究
(In Vitro) | IQ-1 (1.10, 3.48, 11.04 mM; 7 days) maintains the undifferentiated state of ESCs[1].
IQ-1 (0.28, 1.10, 2.76, 11.04 mM; 21 h) maintains murine ESC self-renewal independently of LIF[1].
IQ-1 Binds the PR72/130 Subunit of the Serine/Threonine Phosphatase PP2A in P19 cells[1].
IQ-1 (10 μM; 24 h) modulates Wnt signaling via interaction with PR72/130 in P19 cells[1].
IQ-1 (10 μM; 24 h) indirectly decreases the phosphorylation of p300 Ser-89 and Thereby the β-Catenin/p300 interaction[1].
Cell Viability Assay[1] | Cell Line: | Embryonic stem cells (ESCs) | | Concentration: | 1.10, 3.48, 11.04 mM | | Incubation Time: | 7 days | | Result: | Dose dependently increased alkaline phosphatase activity, in media containing 15% FCS without the addition of exogenous leukemia inhibitory factor (LIF).
Maintained SSEA-1 (undifferentiated ESC marker) expression dose-dependently. |
RT-PCR[1] | Cell Line: | Embryonic stem cells (ESCs) | | Concentration: | 0.28, 1.10, 2.76, 11.04 mM | | Incubation Time: | 21 h | | Result: | Significantly increased and maintained Nanog expression in culture. (Nanog: a divergent homeoprotein pluripotency sustaining factor for ESCs that drives ESC self-renewal).
Exhibited the maintenance of murine ESC pluripotency were independent of the LIF/Stat3 pathway. |
Western Blot Analysis[1] | Cell Line: | P19 cells | | Concentration: | 10 μM | | Incubation Time: | 24 h | | Result: | Significantly reduced coimmunoprecipitation of PR72/130 with both PP2A and Nkd.
Binded to PR72/130 subunit of PP2A, and disrupted the PP2A/Nkd complex.
Decreased the affinity of the β-catenin/p300 interaction and increases β-catenin/CBP usage by negatively regulating the phosphorylation of p300 Ser-89. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : ≥ 33 mg/mL(91.05 mM) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 2.7592 mL | 13.7961 mL | 27.5923 mL | | 5 mM | 0.5518 mL | 2.7592 mL | 5.5185 mL | | 10 mM | 0.2759 mL | 1.3796 mL | 2.7592 mL |
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以下溶剂前显示的百
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