2'-Fucosyllactose (2'-FL) 是一种可从母乳中提取的低聚糖。2'-Fucosyllactose 调节 CD14 的表达，缓解结肠炎，调节肠道菌群。2'-Fucosyllactose 刺激 T 细胞增加 IFN-γ 的产生和减少 IL-6, IL-17 和 TNF-α 细胞因子的产生。
| 生物活性 | 2'-Fucosyllactose (2'-FL) is an oligosaccharide that could be derived from human milk. 2'-Fucosyllactose regulates the expression ofCD14, alleviates colitis and regulates the gut microbiome. 2'-Fucosyllactose stimulates T cells to increaseIFN-γproduction and decreasesIL-6,IL-17, and TNF-α production of cytokines[1][2]. |
| IC50& Target | Target: IFN-γ, IL-6, IL-17, and TNF-α[1] |
体外研究
(In Vitro) | 2'-Fucosyllactose (2'-FL; 0-12 mg/mL; 48 h) suppress cell-associated CD14 expression and to attenuate LPS (100 μg/mL) stimulated IL-8 secretion in T84 cells[1].
2'-Fucosyllactose (2 mg/mL; 48 h; T84 and HCT8 cells) ameliorates inflammation induced by bacterial invasion. 2'-Fucosyllactose inhibits ETEC invasion and attenuated the consequent IL-8 secretion[1].
2'-Fucosyllactose (2 mg/mL; 48 h; T84 and HCT8 cells) induces macrophage migration inhibitory factor signal pathways that suppress inflammation[1].
Western Blot Analysis[1] | Cell Line: | T84 cells | | Concentration: | 0, 2, and 4 mg/mL | | Incubation Time: | 48 hours | | Result: | Suppressed CD14 mRNA and reduced cell-associated CD14 protein expression. |
Western Blot Analysis[1] | Cell Line: | T84 and HCT8 cellsT84 and HCT8 cells | | Concentration: | 2 mg/mL | | Incubation Time: | 48 hours | | Result: | Suppressed CD14 mRNA and reduced cell-associated CD14 protein expression.
Inhibited CD14 and NF-κB induction.
Induced iκB and SOCS2 expression and STAT3 phosphorylation.
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体内研究
(In Vivo) | 2'-Fucosyllactose (2'-FL; 100 mg (200 μL); i.g.; daily, for 4 d; C57BL/6 mice with AIEC infection) inhibits AIEC infection and inflammation in vivo[1].
| Animal Model: | C57BL/6 mice (8 weeks) with AIEC (uropathogenicE. coli, and adherent-invasiveE. coli) infection[1] | | Dosage: | 100 mg (200 μL) | | Administration: | Oral gavage; daily, for 4 days | | Result: | Had colons lengths were closer to normal.
Inhibited the colonisation of the colonic mucosa by O83-positive bacteria.
Decreased CD14 expression, CD14 mRNA levels, IL-6, IL-17 and TNF-α levels in colonic.
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| 结构分类 | - Saccharides
- Polysaccharides
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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