Hesperidin (Hesperetin 7-rutinoside) 是一种广泛存在于柑橘类水果中黄烷酮糖苷。Hesperidin 具有多种生物学特性，例如减少炎症介质并发挥重要的抗氧化作用。Hesperidin 还表现出抗肿瘤和抗过敏活性。
| 生物活性 | Hesperidin (Hesperetin 7-rutinoside), a flavanone glycoside, is isolated from citrus fruits. Hesperidin has numerous biological properties, such as decreasing inflammatory mediators and exerting significant antioxidant effects. Hesperidin also exhibits antitumor and antiallergic activities[1][2]. |
体外研究
(In Vitro) | Hesperidin (5-200 μM; 24-72 h) induces potent cytotoxic effects in human osteosarcoma MG-63 cells[1].
Hesperidin (5-150 μM; 48 h) induces early and late apoptosis in MG-63 cells[1].
Hesperidin (10-30 μM) inhibits the activity of COX-2 and iNOS in a dose dependent manner in RAW 264.7 cells activated with LPS[2].
Hesperidin (0.1 μg/mL; 2 h) decreases the formation of MDA and intracellular ROS, including chondrocyte apoptosis[3].
Cell Viability Assay[1] | Cell Line: | MG-63 cells | | Concentration: | 0, 5, 25, 50, 100, 150, 200 μM | | Incubation Time: | 24, 48, 72 hours | | Result: | Led to time-dependent and concentration-dependent cytotoxic effects, with IC50s of 94.3, 78.6 and 63.3 μM at 24, 48 and 72 h, respectively. |
Apoptosis Analysis[1] | Cell Line: | MG-63 cells | | Concentration: | 0, 5, 50, 150 μM | | Incubation Time: | 48 hours | | Result: | Increased the percentage of apoptotic cells from 4.7% to 17.9, 34.6 and 68.3% at the concentration of 0, 5, 50 and 150 μM, respectively. |
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体内研究
(In Vivo) | Hesperidin (5-80 mg/kg; 2 weeks) significantly suppresses MG-63 tumor growth in mice[1].
Hesperidin (200 mg/kg; once daily for 28 d) markedly attenuates cartilage destruction and reduces IL-1β and TNF-α levels in a surgically-induced osteoarthritis (OA) rats[3].
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : ≥ 33 mg/mL(54.05 mM) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 1.6378 mL | 8.1892 mL | 16.3784 mL | | 5 mM | 0.3276 mL | 1.6378 mL | 3.2757 mL | | 10 mM | 0.1638 mL | 0.8189 mL | 1.6378 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (4.09 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (4.09 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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