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WAY-600
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
WAY-600图片
CAS NO:1062159-35-6
包装:2mg, 5mg, 10mg, 50mg
包装与价格:
包装价格(元)
Free Sample (0.5-1 mg)电议
2mg电议
5mg电议
10mg电议
50mg电议

产品介绍

生物活性

WAY-600是一种有效的,ATP竞争性的,选择性的mTOR抑制剂,IC50为9 nM;抑制mTORC1/P-S6K(T389)和mTORC2/P-AKT(S473),但不抑制P-AKT(T308);作用于mTOR比作用于PI3Kα(>100倍)和PI3Kγ(>500倍)选择性高。


化学数据

分子量494.59
分子式C28H30N8O
CAS号1062159-35-6
纯度99.23%
溶解性(25°C)DMSO ≥ 21 mg/mL
储存和运输条件固体粉末: -20°C 冷藏长期储存
常温运输及临时存放

实验操作 来自于公开的文献,仅供相同实验参考(如实验材料、目的不同,请参考其他文献)

细胞实验
细胞系MDA361 and U87MG cells
方法Cell lines of Rat1, HEK293, MDA-MB-361, MDA-MB-468, MDA-MB-231, LNCap, DU145, U87MG, A498, HCT116, and HT29 were obtained from the American Type Culture Collection. PC3MM2 was obtained from Dr. Carolyn Discafani (Wyeth Discovery Oncology). All cells were cultured using standard cell culture methods. For tumor cell growth assays, cells were plated in 96-well culture plates at 1000 to 3000 cells per well for 24 h, treated with DMSO or various doses of mTOR inhibitors. Viable cell densities were determined three days later by MTS assay employing an assay kit (CellTiter 96(R) AQueous, Cat # G5421, Promega) following the kit assay protocol. The effect of each treatment was calculated as percent of control growth relative to the DMSO-treated cells grown in the same culture plate. Inhibitor dose response curves were plotted for determination of IC50 values.
浓度0.01~100 μM
处理时间3 days

动物实验
动物模型PC3MM2 tumors xenograft model in BALB/c nu/nu female mice
配制5% ethanol, 5% polysorbate 80, 5% polyethylene glycol-400
剂量50mg/kg on days 0 to 4 once daily or twice daily
给药处理i.p.

不同实验动物依据体表面积的等效剂量转换表(数据来源于FDA指南)

小鼠大鼠豚鼠仓鼠
重量 (kg)0.020.151.80.40.0810
体表面积 (m2)0.0070.0250.150.050.020.5
Km系数36128520
动物 A (mg/kg) = 动物 B (mg/kg) × 动物 B的Km系数
动物 A的Km系数

例如,依据体表面积折算法,将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量,需要将20 mg/kg 乘以小鼠的Km系数(3),再除以大鼠的Km系数(6),得到化合物用于大鼠的等效剂量为10 mg/kg。


储备液配制

以下数据基于产品分子量,对于特殊产品,请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass1 mg5 mg10 mg
1 mM2.0219 mL10.1094 mL20.2188 mL
5 mM0.4044 mL2.0219 mL4.0438 mL
10 mM0.2022 mL1.0109 mL2.0219 mL