| 包装: | 1mg |
| 市场价: | 3318元 |
Ghrelin (rat), a growth hormone-releasing peptide first discovered in rat stomach in 1999, is a ligand for the growth hormone secretagogue receptor.
Cell lines | RP adipocytes |
Preparation Method | After isolation, adipocytes were seeded (800 µl of suspension per well) onto 24 well plates; then, 200 µl of the following test solutions were added: culture medium either alone (basal) or containing insulin; dexamethasone phosphate, Ghrelin (rat)(0.001-1 nM), desacyl Ghrelin (rat), cycloheximid, actinomycin D , Ghrelin (rat) antagonist or, when appropriate, different combinations of these substances. |
Reaction Conditions | 0.001-1 nM for 0.5-48h at 37°C |
Applications | Retroperitoneal (RP) adipocytes were cultured in the absence or presence of either Ghrelin (rat) or desacyl Ghrelin (rat) and in combination with either inhibitors of protein synthesis, insulin, dexamethasone (DXM), or GHSR1a antagonist. The results indicate that both Ghrelin (rat) forms possess a direct leptin-releasing activity (LRA) on RP adipocytes and significantly enhanced adipocyte ob mRNA expression. |
Animal models | Male Sprague-Dawley rats (M&B, Ry, Denmark) 12-16 wk of age and weighing ~300 g |
Preparation Method | Ghrelin (rat) (100 nmol/kg) dissolved in saline, was injected into the catheterized femoral vein. The influence of both exogenously administered Ghrelin (rat) and antagonism of endogenous Ghrelin (rat) levels (by GHRP-6 administration) was investigated in normal-fed rats. |
Dosage form | 100 nmol/kg Ghrelin (rat)(I.V) |
Applications | Ghrelin (rat) and the GHS-R1α receptor antagonist GHRP-6 were injected intravenously in rats followed by blood flow measurements using a microsphere technique. Ghrelin decreased, while GHRP-6 in fasted, but not fed, rats selectively increased islet blood flow fourfold. |
| 产品描述 | Ghrelin (rat), a growth hormone-releasing peptide first discovered in rat stomach in 1999, is a ligand for the growth hormone secretagogue receptor[1，2]. Retroperitoneal (RP) adipocytes were cultured in the absence or presence of either Ghrelin (rat) or desacyl Ghrelin (rat) and in combination with either inhibitors of protein synthesis, insulin, dexamethasone (DXM), or GHSR1a antagonist. The results indicate that both Ghrelin (rat) forms possess a direct leptin-releasing activity (LRA) on RP adipocytes and significantly enhanced adipocyte ob mRNA expression[4]. Ghrelin (rat) and the GHS-R1α receptor antagonist GHRP-6 were injected intravenously in rats followed by blood flow measurements using a microsphere technique. Ghrelin (rat) decreased, while GHRP-6 in fasted, but not fed, rats selectively increased islet blood flow fourfold[3]. The initial electrophysiological results displayed that ex vivo administration of Ghrelin (rat) increased NAc shell output in brain slices from drug- and training-naIve rats. In rats with an acquired skilled reach performance, acute as well as repeated treatment with a Ghrelin (rat) receptor (GHSR-1 A) antagonist decreased the number of sucrose pellets consumed[5]. Ghrelin (rat) administration attenuated sepsis symptoms induced by CLP. Blood flow in the stomach greater curvature was significantly higher in the CLP induced sepsis group rats compared with the sham operation group, whereas there was no difference between the CLP group treated with Ghrelin (rat) and the sham rats. Ghrelin (rat) administration also reduced the secretion of pro inflammatory cytokines compared with the CLP induced sepsis group rats[6]. Ghrelin (rat) signaling increases the reward from social interaction in a manner that reflects the degree of divergence in body weight between the social pair[7]. References: |
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