W146 TFA

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W146 TFA

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

909725-62-8

包装与价格：

包装	价格(元)
1mg	电议
5mg	电议
10mg	电议
50mg	电议
100mg	电议

产品介绍

W146 TFA 是 1-磷酸-鞘氨醇受体亚型 1 (S1PR1) 的选择性拮抗剂，EC₅₀值为 398 nM。

生物活性

W146 TFA is a selective antagonist of sphingosine-1-phosphate receptor 1 (S1PR1) with an EC₅₀value of 398 nM.

IC₅₀& Target

EC50: 398 nM (S1PR1)^[1].

体外研究
(In Vitro)

W146 is a S1PR1 antagonist with a K_iof ~70-80 nM^[1].
W146 pretreatment significantly increases activated cleaved caspase-3 levels. The reduced EPCs apoptosis which induced by S1P is completely abolished after treatment with W146^[2].

Apoptosis Analysis^[2]

Cell Line:	Endothelial progenitor cells (EPCs).
Concentration:	10 μM.
Incubation Time:	30 min before the addition of S1P.
Result:	Increases activated cleaved caspase-3 levels.

体内研究
(In Vivo)

W146 (5 mg/kg, ip, prior to AMD3100 administration) pre-treatment shows approximately 8-fold increase of KSL-HSPC mobilization, measured by the CFU-G/M colony forming assays, compared to that in mice treated with AMD3100 alone^[3]
The W146-mediated augmentation of KSL-HSPC mobilization is specific, because pretreatment of mice with W140 is unable to produce any effect on AMD3100-stimulated KSL-HSPC mobilization. Injections of W146, W140, JTE013, or Cay10444 do not alter the basal WBC count in mice^[3].

Animal Model:	Mice(4-6-week-old)^[3] .
Dosage:	5 mg/kg.
Administration:	IP, 1 hour prior to AMD3100 (ADM) administration.
Result:	Significantly increased in KSL-HSPC mobilization compared to that in mice pretreated with dextran followed by AMD3100 administration.

分子量

456.39

性状

Solid

Formula

C₁₈H₂₈F₃N₂O₆P

CAS 号

909725-62-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:

DMSO : 55 mg/mL(120.51 mM;ultrasonic and warming and heat to 60℃)

Ethanol :< 1 mg/mL (ultrasonic;warming;heat to 60℃)(insoluble)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.1911 mL	10.9555 mL	21.9111 mL
5 mM	0.4382 mL	2.1911 mL	4.3822 mL
10 mM	0.2191 mL	1.0956 mL	2.1911 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。