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Smilagenin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Smilagenin图片
CAS NO:126-18-1
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品介绍
Smilagenin (SMI) 是一种小分子甾体皂苷元,来自知母Anemarrhena asphodeloides和天竺葵Pelargonium hortorum,广泛用于慢性神经退行性疾病的研究。Smilagenin (SMI) 通过增加毒蕈碱受体亚型 1 (M1)-受体的的密度来改善老年大鼠的记忆。 Smilagenin (SMI) 通过刺激脑源性神经营养因子的基因表达减弱 Aβ (25-35) 诱导的神经变性,可用于 AD 的研究。
生物活性

Smilagenin (SMI) is a small-molecule steroidal sapogenin fromAnemarrhena asphodeloidesandPelargonium hortorumwidely used in traditional Chinese medicine for treating chronic neurodegeneration diseases[1]. Smilagenin (SMI) improves memory of aged rats by increasing the muscarinic receptor subtype 1 (M1)-receptor density[2]. Smilagenin (SMI) attenuates Aβ(25-35)-induced neurodegenerationvia stimulating the gene expression of brain-derived neurotrophic factor, may represents a novel therapeutic strategy for AD[3].

IC50& Target

mAChR1

 

Human Endogenous Metabolite

 

体外研究
(In Vitro)

Smilagenin (10 μM; 24 hours) increases SH-SY5Y cell survival compared with Aβ(25-35) intoxicated cells[3].
Smilagenin (10 μM; 24 hours) increases neurotrophic factor (GDNF) and neurotrophic factor (BDNF) mRNA level by promoting CREB phosphorylation in 1-methyl-4-phenylpyridimium (MPP+) treated SH-SY5Y cells[2].

Cell Viability Assay[3]

Cell Line:SH-SY5Y cells
Concentration:10 μM
Incubation Time:24 hours
Result:Elevated the SH-SY5Y cell viability.

RT-PCR[2]

Cell Line:SH-SY5Y cells
Concentration:10 μM
Incubation Time:24 hours
Result:Increased GDNF and BDNF transcription.
体内研究
(In Vivo)

Smilagenin (intragastric administration; 10 or 26 mg/kg, once daily; 60 days) prevents the impairment of dopaminergic neurons in chronic MPTP/probenecid-induced mouse model[2].

Animal Model:MPTP/probenecid-induced mouse model[2]
Dosage:10 or 26 mg/kg
Administration:Intragastric administration; 10 or 26 mg/kg; once daily; 60 days
Result:Ameliorated locomotor ability of MPTP/probenecid-lesioned mice.
分子量

416.64

性状

Solid

Formula

C27H44O3

CAS 号

126-18-1

结构分类
  • Steroids
来源
  • Plants
  • Geraniaceae
  • Pelargonium hortorumBailey
  • Plants
  • Liliaceae
  • Anemarrhena asphodeloidesBunge
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

Ethanol : ≥ 10 mg/mL(24.00 mM)

DMSO : 5 mg/mL(12.00 mM;Need ultrasonic)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.4002 mL12.0008 mL24.0015 mL
5 mM0.4800 mL2.4002 mL4.8003 mL
10 mM0.2400 mL1.2001 mL2.4002 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。