NMDA(N-Methyl-D-aspartic acid)

Molecular Weight (MW)	147.13
Formula	C5H9NO4
CAS No.	6384-92-5
Storage	-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)	DMSO: 5 mg/mL (33.98 mM)
Water: 30 mg/mL (203.9 mM)
Ethanol: <1 mg/mL
Solubility (In vivo)	Chemical Name: N-Methyl-D-aspartic acid InChi Key: RVHOBHMAPRVOLO-SCSAIBSYSA-N InChi Code: InChI=1S/C6H10O4/c1-2-4(6(9)10)3-5(7)8/h4H,2-3H2,1H3,(H,7,8)(H,9,10)/t4-/m1/s1 SMILES: O=C(O)C[C@H](C(O)=O)CC
Synonyms	LC488A; LC488 A; LC488-A; LC 488A; LC-488A

Molecular Weight (MW)

147.13

Formula

C5H9NO4

CAS No.

6384-92-5

Storage

-20℃ for 3 years in powder form

-80℃ for 2 years in solvent

Solubility (In vitro)

DMSO: 5 mg/mL (33.98 mM)

Water: 30 mg/mL (203.9 mM)

Ethanol: <1 mg/mL

Solubility (In vivo)

Chemical Name: N-Methyl-D-aspartic acid

InChi Key: RVHOBHMAPRVOLO-SCSAIBSYSA-N

InChi Code: InChI=1S/C6H10O4/c1-2-4(6(9)10)3-5(7)8/h4H,2-3H2,1H3,(H,7,8)(H,9,10)/t4-/m1/s1

SMILES: O=C(O)C[C@H](C(O)=O)CC

Synonyms

LC488A; LC488 A; LC488-A; LC 488A; LC-488A

In Vitro	In vitro activity: NMDA is an excitatory amino acid neurotransmitter, which only binds to the NMDA receptor without effecting other glutamate receptors (such as those for AMPA and kainate). NMDA specifically binds to the NR2 subunits of NMDA receptor, and then stimulates the open of non-specific cation channel which can allow the passage of Ca2+ and Na+ into the cell and K+ out of the cell. Activation of the NMDA receptor is able to produce the excitatory postsynaptic potential (EPSP), and trigger the increase of intracellular Ca2+ content which may further take participating in various signaling pathways. NMDA receptor plays a key role in a wide range of physiological (e.g. long-term potentiation and neuronal plasticity) and pathological processes (e.g. excitotoxicity and epilepsy). Kinase Assay: Adrenal membranous homogenate suspensions are incubated with 10 nM [3H]Glu in 500/zl 50 mM Tris-acetate buffer (pH 7.4) at 2°C or 30°C in the presence and absence of various compounds. Incubation is terminated by the addition of 3 mL ice-cold buffer and subsequent filtration through a Whatman GF/B glass filter under a constant vacuum of 15 mm Hg. After washing the filter 4 times with 3 mL icecold buffer, the radioactivity trapped on the filter is measured by a liquid scintillation spectrometer using 5 mL modified Triton-toluene scintillant at a counting efficiency of 40-42%. The radioactivity found in the presence of 1 mM non-radioactive Glu is subtracted from each experimental value to obtain the specific binding of [3H]GIu in accordance with the y-aminobutyric acid (GABA) receptor binding assay system. The kinetic parameters of [3H]GIu binding, Kd and Bma x, are calculated by Scatchard analysis of the specific binding using a personal computer with a programme for non-linear regression analysis developed in our own laboratory.
In Vivo	NMDA (0.2 nM) shows significant effects on MF, IF, IL, and EL, respectively, decreasing the mount and intromission frequencies, and shortening the intromission and ejaculation latencies. NMDA and AP-5 significantly, respectively, facilitates and inhibits the ejaculatory behavior during the copulation testing 30 min. Bilateral microinjection of NMDA into PVN significantly increases the baseline LSNA, the peaking increment of LSNA occurred within 5 min from the time of NMDA microinjected into PVN
Animal model	Rats: Thirty male rats are paired with different receptive females for a total of three times (once every 3 days) a week prior to the experiment, only the males that ejaculated at least three times during this period are included. After selecting the male rats with normal ejaculatory ability. Saline (100 nL), NMDA (0.20 nmol in 100 nL saline), and AP-5 (10.0 nmol in 100 nL saline) are adminitration into the bilateral PVN of each male rat in random order. After 5 min, the behavioral testing is performed and recorded as described above. Copulatory behaviors occur once a week and the entire experiment lasted 4 weeks.
Formulation & Dosage	0.20 nmol in 100 nL saline
References	Curr Opin Neurobiol. 2001 Jun;11(3):327-35.

In Vitro

In vitro activity: NMDA is an excitatory amino acid neurotransmitter, which only binds to the NMDA receptor without effecting other glutamate receptors (such as those for AMPA and kainate). NMDA specifically binds to the NR2 subunits of NMDA receptor, and then stimulates the open of non-specific cation channel which can allow the passage of Ca2+ and Na+ into the cell and K+ out of the cell. Activation of the NMDA receptor is able to produce the excitatory postsynaptic potential (EPSP), and trigger the increase of intracellular Ca2+ content which may further take participating in various signaling pathways. NMDA receptor plays a key role in a wide range of physiological (e.g. long-term potentiation and neuronal plasticity) and pathological processes (e.g. excitotoxicity and epilepsy).

Kinase Assay: Adrenal membranous homogenate suspensions are incubated with 10 nM [3H]Glu in 500/zl 50 mM Tris-acetate buffer (pH 7.4) at 2°C or 30°C in the presence and absence of various compounds. Incubation is terminated by the addition of 3 mL ice-cold buffer and subsequent filtration through a Whatman GF/B glass filter under a constant vacuum of 15 mm Hg. After washing the filter 4 times with 3 mL icecold buffer, the radioactivity trapped on the filter is measured by a liquid scintillation spectrometer using 5 mL modified Triton-toluene scintillant at a counting efficiency of 40-42%. The radioactivity found in the presence of 1 mM non-radioactive Glu is subtracted from each experimental value to obtain the specific binding of [3H]GIu in accordance with the y-aminobutyric acid (GABA) receptor binding assay system. The kinetic parameters of [3H]GIu binding, Kd and Bma x, are calculated by Scatchard analysis of the specific binding using a personal computer with a programme for non-linear regression analysis developed in our own laboratory.

In Vivo

NMDA (0.2 nM) shows significant effects on MF, IF, IL, and EL, respectively, decreasing the mount and intromission frequencies, and shortening the intromission and ejaculation latencies. NMDA and AP-5 significantly, respectively, facilitates and inhibits the ejaculatory behavior during the copulation testing 30 min. Bilateral microinjection of NMDA into PVN significantly increases the baseline LSNA, the peaking increment of LSNA occurred within 5 min from the time of NMDA microinjected into PVN

Animal model

Rats: Thirty male rats are paired with different receptive females for a total of three times (once every 3 days) a week prior to the experiment, only the males that ejaculated at least three times during this period are included. After selecting the male rats with normal ejaculatory ability. Saline (100 nL), NMDA (0.20 nmol in 100 nL saline), and AP-5 (10.0 nmol in 100 nL saline) are adminitration into the bilateral PVN of each male rat in random order. After 5 min, the behavioral testing is performed and recorded as described above. Copulatory behaviors occur once a week and the entire experiment lasted 4 weeks.

Formulation & Dosage

0.20 nmol in 100 nL saline

References

Curr Opin Neurobiol. 2001 Jun;11(3):327-35.

CAS NO:	6384-92-5
规格:	≥98%

包装	价格(元)
50mg	电议
100mg	电议
250mg	电议
500mg	电议
1g	电议
2g	电议