Prodigiosin (Prodigiosine) hydrochloride，一种天然的红色素，是一种具有生物活性的次生代谢产物。Prodigiosin hydrochloride 是一种有效的促凋亡剂，可抑制Wnt/β-catenin通路。Prodigiosin hydrochloride 具有抗菌，抗真菌，抗原生动物，抗疟疾，免疫抑制和抗癌特性。
| 生物活性 | Prodigiosin (Prodigiosine) hydrochloride is a red pigment produced by bacteria as a bioactive secondary metabolite. Prodigiosin hydrochloride is a potent proapoptotic agent, and inhibitsWnt/β-cateninpathway. Prodigiosin hydrochloride has antibacterial, antifungal, antiprotozoal, antimalarial, immunosuppressive, and anticancer properties[1][2]. |
体外研究
(In Vitro) | Prodigiosin (25-500 nM; 24 hours) treatment reduces the viability of breast cancer cells, with IC50values at 48 h of 62.52 nM in MDA-MB-231 cells and 261.2 nM in MDA-MB-468 cells[1].
Prodigiosin (25-500 nM; 24 hours) treatment significantly reduces the levels of phosphorylated LRP6 and DVL2, active β-catenin, and total β-catenin. Prodigiosin noticeably inhibits the phosphorylation of GSK3β at Ser9 in HEK293T cells, which is indicative of an increase in GSK3β activity[1].
Prodigiosin can inhibit proliferation and induce apoptosis in breast cancer cells[1].
Prodigiosin (25-500 nM; 24 hours) treatment dose-dependently blocks Wnt signaling activated by Wnt1, Wnt3, Wnt1/LRP6, Wnt3/LRP6, and Dishevelled 2 (DVL2) in transfected HEK293T cells. Prodigiosin treatment inhibits Wnt3A-CM-induced transcription in a dose-dependent manner. Prodigiosin inhibits transcription of the SuperTopFlash reporter activated by either Wnt transfection or Wnt3A treatment[1].
When applied to cultures of chytrid fungiBatrachochytrium dendrobatidisandB. salamandrivorans, Prodigiosin causes significant growth inhibition, with MIC values of 10 μM and 50 μM, respectively[2].
Cell Proliferation Assay[1] | Cell Line: | MDA-MB-231 and MDA-MB-468 cells | | Concentration: | 10 nM, 25 nM, 50 nM, 100 nM, 250 nM, 500 nM, 1000 nM, 2500 nM, 5000 nM | | Incubation Time: | 24 hours, 48 hours | | Result: | Reduced the viability of breast cancer cells, with IC50 values at 48 h of 62.52 nM in MDA-MB-231 cells and 261.2 nM in MDA-MB-468 cells. |
Western Blot Analysis[1] | Cell Line: | HEK293T cells | | Concentration: | 50 nM, 100 nM, 250 nM, 500 nM | | Incubation Time: | 24 hours | | Result: | Significantly reduced the levels of phosphorylated LRP6 and DVL2, active β-catenin, and total β-catenin. |
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体内研究
(In Vivo) | Prodigiosin (5 mg/kg; intraperitoneal injection; twice weekly; for 3 weeks) treatment significantly inhibits tumor growth. Prodigiosin treatment decreases tumor cell density and expression of the proliferation marker Ki-67[1].
| Animal Model: | Female BALB/c nude mice injected with MDA-MB-231 cells[1] | | Dosage: | 5 mg/kg | | Administration: | Intraperitoneal injection; twice weekly; for 3 weeks | | Result: | Significantly inhibited tumor growth in mice. |
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| 结构分类 | - Alkaloids
- Pyrrole Alkaloids
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | -20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
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