HBC514

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HBC514

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

2530161-67-0

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
5mg	电议
10mg	电议
25mg	电议
50mg	电议
100mg	电议
200mg	电议

产品介绍

HBC514 本身是一种非荧光的 HBC 模拟物，但在与 Pepper RNA 适配体形成紧密复合物后可以发出强烈的绿色荧光，用于RNA标记。在活的 Pepper 标记的细胞中，HBC514-Pepper complex 可以使RNAs可视化，并且可以通过简单的洗涤和染色灵活地改变荧光。

生物活性

HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells[1].

IC₅₀& Target

IC50: RNA fluorescent dye

体外研究
(In Vitro)

HBC is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor, nonfluorescent in solution. ‘Pepper’ is able to enhance HBC’s fluorescence more than 3,000-fold and exhibits bright fluorescence when expressed in living cells^[1].
‘Pepper’ has reduced Mg²⁺sensitivity after multiple rounds of optimization, displays a well-defined fluorescent profile with an excitation maximum at 485nm, an emission maximum at 530nm, and excellent brightness^[1].
HBC514 (2-10 μM) is altered flexibly by simple washing and staining by Pepper based CRISPR imaging in live HeLa cells (expressing sgRNA-Pep1 and dCas9)^[1].

分子量

330.43

性状

Solid

Formula

C₂₁H₂₂N₄

CAS 号

2530161-67-0

Emission (Em)

Em 495-570 nm Green

Excitation (Ex)

Ex 495-570 nm Green

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 25 mg/mL(75.66 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	3.0264 mL	15.1318 mL	30.2636 mL
5 mM	0.6053 mL	3.0264 mL	6.0527 mL
10 mM	0.3026 mL	1.5132 mL	3.0264 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。