Cyanine5 NHS ester bromide is a active compound, can be used to label amino groups inpeptides, proteins, andoligonucleotides. Cyanine5 NHS ester bromide is a cyanine dye, fluorescence-labeling neurotensin (8-13) via arginine residues^[1].

Cyanine5 NHS ester bromide can be excitable with a red (635 nm) and a 488 nm argon laser, respectively^[1].
Cyanine5 NHS ester bromide applys for characterization^[1]:
1. Acquire high resolution mass spectra (HRMS) with Agilent 6540 UHD Accurate-Mass QTOF LC/MS system coupled to an Agilent 1290 HPLC system, using an ESI source.
2. Perform preparative HPLC with a system from Knauer (Berlin, Germany). A Kinetex-XB C18, 5 μm, 250 × 21 mm (Phenomenex, Aschaffenburg, Germany) is used as stationary phase. Mixtures of 0.1% aq TFA and acetonitrile are used as mobile phase. The flow rate is 18-20 mL/min, and a detection wavelength of 220 nm is used throughout.
3. Analysis HPLC with a system from Agilent Technologies. A Kinetex-XB C18, 2.6 μm, 100 × 3 mm (Phenomenex) serves as stationary phase at a flow rate of 0.5-0.6 mL/min. The oven temperature is set to 25 ℃. Mixtures of 0.04% aq TFA and acetonitrile are used as mobile phase. The injection volume is 20 μL. Detection is performed at 220 nm.

660.64

C₃₆H₄₂BrN₃O₄

1653991-59-3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.