4-Methylumbelliferyl phosphate (4-MUP) disodium, an anionic organophosphate, is a acid and alkalinephosphatasefluorogenic substrate. 4-Methylumbelliferyl phosphate disodium is also a nerve agent simulant^[1][2][3].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Serum acid phosphatase assay^[1]:
1. Preparation a 100 μL solution containing the following agents: 5.0 μL of serum enzyme, 50 μL of 4-Methylumbelliferyl phosphate (5.0 mM), and 10 μL of 1.0 M buffer at pH 6.0.
2. Add the following agents (with the final concentration) 20 mMSodium Tartrate(HY-128476), 15 mMSodium Fluoride(HY-B1766), 3000 mM 2-Mercaptoethanol, 4 mM1, 10-Phenanthroline(HY-W004544), 6 mM8-Quinolinol(HY-B1005), 6 mM 8-Hydroxy-5quinolinesulfonic acid, 45 mMEDTA(HY-Y0682) and 6 mMEGTA(HY-D0861).
3. Incubate reaction mixtures for 15 min at 37℃ and terminated by the addition of 2.9 mL of 0.1 M ammonium hydroxide/glycine buffer, pH 10.5.
4. Mix samples thoroughly and determine fluorescence by a fluorometer.

300.11

C₁₀H₇Na₂O₆P

22919-26-2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.