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5-FAM SE
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
5-FAM SE图片
CAS NO:92557-80-7
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
10mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品介绍
5-FAM SE 是一种单一异构体,是一种用于标记肽,蛋白质和核苷酸的荧光标记试剂。5-FAM SE 可以与胺反应,并可以产生稳定的胺结合物。
生物活性

5-FAM SE is a single isomer, is a fluorescent labeling reagent used for labelingpeptides, proteins and nucleotides. 5-FAM SE can react with amines and can yield stable amine conjugates[1][2].

体外研究
(In Vitro)

The capillary electrophoresis (CE)-laser-induced fluorescent (LIF) method with 5-FAM SE (5-Carboxyfluorescein N-succinimidyl ester) derivatization for the analysis of serotonin can be used to quantify luminally released 5-HT with high sensitivity. CFSE is an excellent dye for the derivatization of 5-HT[1].
5-FAM SE (5-Carboxyfluorescein succinimidyl ester) is chosen as the labeling reagent to precapillary derivatize the two marker aminohydroxyphenylalanine (AHP) isomers produced after reductive hydrolysis of pheomelanin with hydriodic acid (HI)[2].

分子量

473.39

性状

Solid

Formula

C25H15NO9

CAS 号

92557-80-7

Emission (Em)
Em 495-570 nm Green
Excitation (Ex)
Ex 495-570 nm Green
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro: 

DMSO : 25 mg/mL(52.81 mM;Need ultrasonic and warming)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.1124 mL10.5621 mL21.1242 mL
5 mM0.4225 mL2.1124 mL4.2248 mL
10 mM0.2112 mL1.0562 mL2.1124 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light, stored under nitrogen)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。