FD-1080 是在 NIR-II 区域中具有激发和发射光的荧光团 (Ex=1064 nm, Em=1080 nm)，可用于体内成像。
| 生物活性 | FD-1080 is a fluorophore with both excitation and emission in the NIR-II region (Ex=1064 nm, Em=1080 nm). FD-1080 can be used for in vivo imaging[1]. |
体外研究
(In Vitro) | 1. FD-1080 工作液的配制
1.1 制备储存液
用 DMSO 稀释 FD-1080 配制 10 mM 的 FD-1080。
注：FD-1080 储存液建议分装后于 -20 ℃ 或 -80 ℃ 避光保存。
1.2 工作液的配制
用预热好的PBS 稀释储存液，配制成 2-10 μM 的 FD-1080 工作液。0.2 μm 滤膜过滤除菌。
注：请根据实际情况调整 FD-1080 工作液浓度，且现用现配。
2. 细胞实验
2.1 悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。
贴壁细胞：弃去培养基，加入胰蛋白酶消化细胞。离心弃去上清后，加入 PBS 洗涤两次，每次 5 分钟。
2.2 加入 1 mL FD-1080 工作液，室温孵育 10-30 分钟。
2.3 400 g，4℃ 离心 3-4 分钟，弃去上清。
2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。
2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，在荧光显微镜或流式细胞仪下检测。
3. 活体注射
3.1 将 200 μL 80 μM FD-1080工作液静脉注射至小鼠体内，10-20 分钟后进行体内成像分析。
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体内研究
(In Vivo) | The 1064 nm NIR-II excitation of FD-1080 is demonstrated with the high tissue penetration depth and superior imaging resolution compared to NIR excitation from 650 nm to 980 nm. Deeptissue and high-resolution in vivo imaging for the left hindlimb vasculature, abdomen, and brain vessels was realized, allowing penetration through intact skin, tissue, and skull. FD-1080 also quantifying the respiratory rate based on the dynamic imaging of respiratory craniocaudal motion of the liver for the awake and anaesthetized mouse[1].
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| 分子量 | |
| 性状 | |
| Formula | |
| CAS 号 | |
| Emission (Em) | Em >750 nm Infrared |
| Excitation (Ex) | Ex >750 nm Infrared |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 染色示例 | Description: FD-1080 can be used as a fluorophore for preparing 1080 nm NIR-triggered liposomes.Method: For preparing 1080 nm NIR-triggered liposomes.1. Mix FD-1080 (0.1 mg), phosphatidylcholine (20 mg), cholesterol (5 mg) and dissolve in the solution of chloroform/methanol (5:1) overnight at room temperature.2. Dry solution with the organic solvent at 37℃ with a rotary evaporator to form a uniform liposome film on the wall of the eggplant flask, and further blow-dried with nitrogen for 5 min to fully volatilize the residual organic solvent.3. Add PBS (0.5 M; 5 mL; pH=6.5) with liposome, and disperse into a uniform and stable transparent liquid with shaker and ultrasonic (70 Hz, 5 min) until the membrane is fully dissolved.4. Use an electron microscope for image.
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