3',4'-Dimethoxyflavone 是一种亲脂性黄酮，可分离自黄花九轮草 (Primula veris) 的叶子。3',4'-Dimethoxyflavone 可减少PARP的合成和积累，保护皮质神经元免受Parthanatos 通路诱导的细胞死亡。3',4'-Dimethoxyflavone 在人乳腺癌细胞中也是一种芳香烃受体 (aryl hydrocarbon receptor) 拮抗剂。3',4'-Dimethoxyflavone 能促进人体造血干细胞增殖。具有多种生物活性，包括抗氧化、抗癌、抗炎、抗动脉粥样硬化、降血脂和神经保护或神经营养作用。
| 生物活性 | 3',4'-Dimethoxyflavone is a lipophilic flavone, can be isolated from the leaves ofPrimula veris. 3',4'-Dimethoxyflavone can reduce the synthesis and accumulation ofPARPand protect cortical neurones against cell death induced by Parthanatos. 3',4'-Dimethoxyflavone is also anaryl hydrocarbon receptorantagonist in human breastcancercells. 3',4'-Dimethoxyflavone can promote the proliferation of human hematopoietic stem cells. 3',4'-Dimethoxyflavone has various biological activities, including antioxidant, anti-cancer, anti-inflammatory, anti-atherogenic, hypolipidaemic, and neuroprotective or neurotrophic effects[1][2][3][4]. |
| IC50& Target | PARP, Aryl hydrocarbon receptor[1] |
体外研究
(In Vitro) | 3',4'-Dimethoxyflavone (10 and 20 μM) has protection against the reduction in SH-SY5Y viability induced byMethylnitronitrosoguanidine(MNNG) (HY-128612)[2].
3',4'-Dimethoxyflavone (6.25-25 μM) decreases the levels of PAR induced by MNNG in HeLa cells[2].
3',4'-Dimethoxyflavone (12.5, 25, 50 and 100 μM; 15-20 h) reduces cortical neuronal death induced by exposure toNMDA(HY-17551)[2].
3',4'-Dimethoxyflavone (0.1-10 μM; 24 h) exhibits significant inhibition of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)-induced EROD activity in MCF-7 and T47D cells[3].
3',4'-Dimethoxyflavone inhibits AhR-dependent CYP1A1 induction and AhR-mediated inhibition of estrogen-induced gene expression in T47D and MCF-7 breast cancer cells[3].
3′,4′-Dimethoxyflavone (2.5 μM; 7 days) promotes the proliferation of human hematopoietic stem cells[4].
Cell Viability Assay[2] | Cell Line: | Primary cortical neurones (isolated from fetal CD1 mice, incubated with NMDA) | | Concentration: | 12.5, 25, 50 and 100 μM | | Incubation Time: | 15-20 h | | Result: | Reduced concentration-dependently neuronal death induced by exposure to NMDA. |
Cell Proliferation Assay[4] | Cell Line: | CD34+cells | | Concentration: | 2.5 μM | | Incubation Time: | 7 days | | Result: | Induced a significantly higher amplification of the CD34+population under normoxia. |
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| 来源 | - Plants
- Euphorbiaceae
- Euphorbia hirtaL.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : ≥ 50 mg/mL(177.12 mM) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 3.5425 mL | 17.7123 mL | 35.4246 mL | | 5 mM | 0.7085 mL | 3.5425 mL | 7.0849 mL | | 10 mM | 0.3542 mL | 1.7712 mL | 3.5425 mL |
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储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (8.86 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.86 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.5 mg/mL (8.86 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (8.86 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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