trans-Chalcone 是从Aronia melanocarpa果皮中分离出来的，是类黄酮前体的双酚核心结构。trans-Chalcone 是有效的脂肪酸合酶 (FAS) 和 α-淀粉酶 (α-amylase) 抑制剂。trans-Chalcone 引起细胞周期停滞并诱导乳腺癌细胞系 MCF-7 凋亡。trans-Chalcone 具有抗真菌和抗癌活性。
| 生物活性 | trans-Chalcone, isolated fromAronia melanocarpaskin, is a biphenolic core structure offlavonoidsprecursor. trans-Chalcone is a potentfatty acid synthase(FAS)andα-amylaseinhibitor. trans-Chalcone causes cellcycle arrest and inducesapoptosisin the breastcancer cell line MCF-7. trans-Chalcone hasantifungaland anticancer activity[1][2][3]. |
体外研究
(In Vitro) | trans-Chalcone competitively inhibits porcine pancreatic α-amylase with a Kiof 48 μM[2].
trans-Chalcone (30.23-98.03 μM; 24 hours) induces cell cycle arrest and apoptosis in MCF-7 cells[1].
trans-Chalcone (20-80 μM; 24, 48 hours) reduces the expression of the apoptosis-related protein Bcl-2[1].
trans-Chalcone (58.25 μM; 6, 24 hours) has greater inhibition of Bcl-2, induction of APAF1 and BAX, and strong induction of CIDEA in 24 hours[1].
trans-Chalcone (24 hours) inhibits MCF-7 cell viability (IC20=30.23 μM; IC50=58.25 μM; IC80=98.03 μM). trans-Chalcone (48 h) has IC50s of 41.53 μM and 48.41 μM for MCF-7 and 3T3 cell lines, respectively. trans-Chalcone exhibits a pronounced cytotoxicity activity[1].
Apoptosis Analysis[1] | Cell Line: | MCF-7 cell | | Concentration: | 30.23, 58.25, 98.03 μM | | Incubation Time: | 24 hours | | Result: | Induced apoptosis of the breast cancer cell line. |
Cell Cycle Analysis[1] | Cell Line: | MCF-7 cell | | Concentration: | 30.23, 58.25, 98.03 μM | | Incubation Time: | 24 hours | | Result: | Caused cell cycle arrest in G1. |
Western Blot Analysis[1] | Cell Line: | MCF-7 cell | | Concentration: | 20, 40, 80 μM | | Incubation Time: | 24, 48 hours | | Result: | Reduced the expression of the apoptosis-related protein Bcl-2 and induced the expression of the CIDEA gene.
There was marked degradation of cyclin D1 at 48 h. |
RT-PCR[1] | Cell Line: | MCF-7 cell | | Concentration: | 58.25 μM | | Incubation Time: | 6, 24 hours | | Result: | Had greater inhibition of Bcl-2, induction of APAF1 and BAX, and strong induction of CIDEA in 24 hours. |
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| 来源 | - Plants
- Rosaceae
- Aronia melanocarpa
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : 100 mg/mL(480.17 mM;Need ultrasonic) 配制储备液 | 1 mM | 4.8017 mL | 24.0085 mL | 48.0169 mL | | 5 mM | 0.9603 mL | 4.8017 mL | 9.6034 mL | | 10 mM | 0.4802 mL | 2.4008 mL | 4.8017 mL |
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以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (12.00 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (12.00 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.5 mg/mL (12.00 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (12.00 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 3. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.5 mg/mL (12.00 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (12.00 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。 *以上所有助溶剂都可在本网站选购。
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