您好,欢迎来到试剂信息网! [登录] [免费注册]
试剂信息网
位置:首页 > 产品库 > Fl-DIBO
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
Fl-DIBO
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Fl-DIBO图片
CAS NO:1407523-31-2
包装与价格:
包装价格(元)
5mg电议
10mg电议
50mg电议
100mg电议

产品介绍
Fl-DIBO (fluorogenic dibenzocyclooctyne) 是一种对叠氮化合物 (azide) 具有选择性、高灵敏度的荧光探针。Fl-DIBO 可以与叠氮化合物快速反应形成新的高荧光产物,其最大发射波长为 469 nm,激发波长为 363 nm。Fl-DIBO 可用于标记重氮标签蛋白而无可检测的背景信号干扰。
生物活性

Fl-DIBO (fluorogenic dibenzocyclooctyne) is a selective and high sensitivity fluorescent probe toazidecompounds. Fl-DIBO can react rapidly with azide compounds to form new highly fluorescent products with a maximum emission wavelength of 469 nm and excitation wavelength of 363 nm. Fl-DIBO can be used to label diazo-tagged proteins without detectable background signal interference[1][2].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling diazo-labeled proteins[2][3]:
1. Resuspend the protein with 1×PBS (pH 7.4) and incubate with NHS-activated ester (25 mM in DMSO) overnight at room temperature.
2. Labeled proteins are co-incubated with Fl-DIBO (25-500 μM) at 37 ℃ for 18 h.
3. Protein SDS-PAGE gel analysis and in-gel fluorescence imaging analysis with λex=365 nm, λem=480 nm.

分子量

288.30

性状

Solid

Formula

C19H12O3

CAS 号

1407523-31-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month