您好,欢迎来到试剂信息网! [登录] [免费注册]
试剂信息网
位置:首页 > 产品库 > 8-Bromo-cGMP sodium
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
8-Bromo-cGMP sodium
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
8-Bromo-cGMP sodium图片
CAS NO:51116-01-9
包装与价格:
包装价格(元)
10 mM * 1 mL in Water电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品介绍
8-Bromo-cGMP sodium 是 cGMP 的膜渗透性类似物,是一种PKG激活剂。8-Bromo-cGMP sodium 可显着抑制Ca2+宏观电流并损害高 K+刺激的胰岛素释放。8-Bromo-cGMP sodium 具有缓解疼痛和血管舒张作用。
生物活性

8-Bromo-cGMP sodium, a membrane-permeable analogue of cGMP, is aPKG (protein kinase G)activator. 8-Bromo-cGMP sodium significantly inhibitsCa2+macroscopic currents and impairsinsulinrelease stimulated with high K+[1]. 8-Bromo-cGMP sodium has antinociceptive effects and results in vasodilator responses[2].

体外研究
(In Vitro)

8-Bromo-cGMP sodium (1-100 μM; 8 h) increases resistance of LLC-PK1 cells to CsA toxicity concentration-dependently[3].
8-Bromo-cGMP sodium (1-100 μM; 16 h) induces the synthesis of HO-1 protein in a concentration-dependent fashion[3].

Cell Viability Assay[3]

Cell Line:LLC-PK1 cells (ATCC CL 101)
Concentration:1-100 μM
Incubation Time:8 hours
Result:Increased resistance of LLC-PK1 cells to Cyclosporin A (CsA) toxicity concentration-dependently and augmented cell viability by up to 65%.

Western Blot Analysis[3]

Cell Line:LLC-PK1 cells (ATCC CL 101)
Concentration:1-100 μM
Incubation Time:16 hours
Result:Induced the synthesis of HO-1 protein in a concentration-dependent fashion.
体内研究
(In Vivo)

8-Bromo-cGMP sodium (0.3, 1, 3.0 nmol; intrathecal administration; 10 min before test) dose-dependently and significantly increases the tail-flick latency in Vincristine-treated mice to the level observed in vehicle-treated naive mice (male ICR mice, 4weeks of age and weighing 20 g). Vincristine (0.05 mg/kg 1 day after the pre-drug tail-flick latency, and then 0.125 mg/kg twice a week for 6 weeks) can induce painful neuropathy in mice[4].
8-Bromo-cGMP sodium (10 mg/kg; iv; single dose) results in vasodilator responses in eNOS-Tg mice and WT littermates in C57BL/6 background (19-35 g)[5].

分子量

446.08

性状

Solid

Formula

C10H10BrN5NaO7P

CAS 号

51116-01-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 100 mg/mL(224.18 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.2418 mL11.2088 mL22.4175 mL
5 mM0.4484 mL2.2418 mL4.4835 mL
10 mM0.2242 mL1.1209 mL2.2418 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (224.18 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。