4-oxo-2-Nonenal-d₃(4-ONE-d₃) contains three deuterium atoms at the terminal methyl position. It is intended for use as an internal standard for the quantification of 4-ONE by GC-or LC-mass spectrometry (MS). 4-ONE is a lipid peroxidation product derived from oxidized ω-6 polyunsaturated fatty acids such as arachidonic acid and linoleic acid.^1,2It exhibits various biological activities such as cytotoxicity, growth inhibiting activity, genotoxicity, and chemotactic activity and has been widely used as a marker of lipid peroxidation.^1,2,34-ONE is a more recently identified product of lipid peroxidation.^4,5,6It actively modifies histidine and lysine residues on proteins and causes protein cross-linking.^7,84-ONE also modifies 2'-deoxy guanosine, further implicating lipid peroxidation in mutagenesis and carcinogenesis.¹

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