Alarelin acetate是一种合成的GnRH激动剂。
产品描述
Alarelin acetate is a synthetic GnRH agonist which can directly inhibit proliferation and DNA synthesis of rat GSMC through GnRH receptors.
体外活性
The cell viability in the presence of alarelin was significantly lower compared to in the absence of alarelin. The maximum stimulatory effect on cell viability was achieved at a concentration of 10-5 M and it acted in a dose-dependent manner[1].
体内活性
Alarelin could inhibit the gastric acid secretion both by direct actions on parietal cells in rats and by inhibiting vagous function[2]. Alarelin could markedly increase the ratio of G1 phase and decrease ratio of S phase of GSMC of rats[1].
细胞实验
The cells are trypsinized in a solution of 2.5 g/L trypsin and seeded in a 96-well plate. After the cells are grown for 24 h to approximately 800 g/L subconfluent state, 0.1 mL medium containing 2.5% calf serum and various concentrations (0.001, 0.1, 10 μM) of alarelin is added to each well, respectively, and incubated for 24 h in a CO2 incubator. Each concentration is tested in at least 12 wells. Briefly, 15 μL of MTT solution is added to each well and incubated for 4 h. Then, the medium and MTT are removed and 150 μL of DMSO is added to each well and shaken for 10 min to dissolve the crystal. The OD is determined at 490 nm using an ELISA reader[1].
Cas No.
79561-22-1
分子式
C58H82N16O14
分子量
1227.392
别名
Alarelin;阿拉瑞林
储存和溶解度
DMSO:58 mg/mL
Powder: -20°C for 3 years
In solvent: -80°C for 2 years