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BSJ-03-123
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
BSJ-03-123图片
CAS NO:2361493-16-3
包装与价格:
包装价格(元)
1 mg电议
2 mg电议
5 mg电议
10 mg电议
25 mg电议
50 mg电议
100 mg电议

产品介绍
BSJ-03-123是一种有效的 CDK6 选择性小分子降解剂,是由Cereblon配体和CDK配体相连的PROTAC。

产品描述

BSJ-03-123 is a potent, CDK6-selective small-molecule degrader.

体外活性

BSJ-03-123 induced rapid, dose-dependent ternary complex formation with CDK6 and CRBN, but not with CDK4 and CRBN. Ternary complex formation was prevented by blocking binding sites on CDK6 or CRBN via pretreatment with palbo or lenalidomide.

细胞实验

4x10^6 MV4-11 CRBN-/- cells were treated for 3 h with 20 μM palbociclib, BSJ-03-123 or vehicle. 1x10^6 cells were spun down at 500 x g for 5 min, and the supernatant removed. Pellets were incubated at 46, 49, 52 or 55°C for 3 min followed by 3 min incubation at room temperature. 30 μl of lysis buffer (20 mM Tris-HCl pH 8.0, 120 mM NaCl, 0.5% NP-40, protease inhibitors) were added and cells lysed by 3 rounds of snap freezing and thawing. Denatured proteins were removed by 20 min centrifugation at 14.000 x g at 4°C and supernatants analyzed by Western Blotting.

Cas No.

2361493-16-3

储存和溶解度

DMSO:115 mg/mL (122.73 mM)
H2O:Insoluble
Powder: -20°C for 3 years
In solvent: -80°C for 2 years