AspeROSaponin VI 是一种来自续断壁的皂苷组分。它通过增加 BCL-2/Bax 比值，降低活性 Caspase-3 表达，以及增强 p-Akt 和 p-CREB，从而抑制缺氧诱导的心肌细胞凋亡。它通过 BMP-2/p38 和 ERK1/2 信号途径诱导成骨细胞分化。

Akebia saponin D, the most abundant constituent of the rhizome of Dipsacus asper, has been used for the treatment of lower back pain, traumatic hematoma and bone fractures.

Cells are grown on 12-mm diameter glass coverslips in DMEM 100% serum and various concentrations of KN-93 or KN-92. After 0, 1, 2, and 3 days of culture in the presence of drug, coverslips are removed from culture, rinsed once in PBS, and then submerged in 100% methanol at -20°C for 3 min. Fixed cells are stored in PBS until staining using the TUNEL assay. Cells are overlaid on 20 μL PBS/1 mg/mL BSA for 30 min, rinsed in PBS, and then overlaid on 20 μL containing 100 mM sodium cacodylate (pH 6.8), 1 mM CoCl2, 0.1 mM DTT, 0.1 mg/mL BSA, 20 μM fluorescein-12-dUTP, and 0.1 unit/μL terminal transferase at 37°C for 60 min. Coverslips are rinsed in PBS twice, mounted on slides, and photographed using an OLYMPUS BX5O epifluorescent microscope using a UPLAN APO 40X oil immersion objective.

39524-08-8

C47H76O18

929.107

Akebia saponin D;Asperosaponin Ⅵ;川续断皂苷 VI

DMSO:10 mM
Powder: -20°C for 3 years
In solvent: -80°C for 2 years