Tulipalin A 是来源于郁金香鳞茎的有毒糖苷。Tulipalin A是引起过敏性接触皮炎的致敏性致敏物 (causative allergen)，在低剂量影响免疫细胞的功能，例如 Jurkat T 细胞。

Tulipalin A (2-Methylenebutyrolactone) which has some phytotoxicity is a natural compound extracted from Tulipa gesneriana.

Tulipalin A-induced phytotoxicity is persistent allergic contact dermatitis documented in floral workers exposed to Alstroemeria and its cultivars [1]. THP-1 cells showed strong robustness to Tulipalin A treatment since only five proteins were altered. In contrast, in Jurkat T cells an increase in the abundance of 66 proteins and a decrease of six proteins was determined [2].

The influence of TUPA on cell viability was measured using the MTT assay. Cells were seeded in flat bottom 96-well plates at a density of 4 x 10^5 cells/ml. Afterward, Jurkat cells were exposed to TUPA in concentrations ranging from 10 to 82 μM. THP-1 cells (4 x 10^5 cells / ml) were exposed to concentrations ranging from 20 to 163 μM. After incubation for 72 h under humidified conditions (5 % CO2, 37 °C), 10 μl of MTT (5 mg/ml) per well were added and cells were further incubated for 3 h. Subsequently, 200 μl DMSO was added for cell lysis and formazan solubilization following incubation of the plates for 15 min while shaking. Afterward, absorbance was recorded at 550 nm and 620 nm using a microplate reader. In each plate, cells remained either untreated (negative control) or were exposed to Etoposide (2.5 μM) as a positive control. Using linear regression of the cell viability as a function of the compound concentrations, the concentrations leading to viability decreases of 10 % and 50 %, respectively, were calculated [2].

547-65-9

C5H6O2

98.101

2-Methylenebutyrolactone;2-甲烯基丁内酯;α-methylene-γ-butyrolactone

DMSO:10 mM
Powder: -20°C for 3 years
In solvent: -80°C for 2 years