您好,欢迎来到试剂信息网! [登录] [免费注册]
试剂信息网
位置:首页 > 产品库 > ML167(CID 44968231)
立即咨询
咨询类型:
     
*姓名:
*电话:
*单位:
Email:
*留言内容:
请详细说明您的需求。
*验证码:
 
ML167(CID 44968231)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
ML167(CID 44968231)图片
CAS NO:1285702-20-6
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
250mg电议
500mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)335.36
FormulaC19H17N3O3
CAS No.1285702-20-6
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 67 mg/mL (199.8 mM)
Water: <1 mg/mL
Ethanol: 13 mg/mL (38.8 mM)
SMILESOCC1=CC=C(C2=CC3=C(NCC4=CC=C(C)O4)N=CN=C3C=C2)O1
SynonymsCID 44968231; NCGC00188654; ML167; CID44968231; NCGC 00188654; ML-167; CID-44968231; NCGC-00188654; ML 167
实验参考方法
In Vitro

In vitro activity: ML167 (also known as CID 44968231) is a highly selective Cdc2-like kinase 4 (Clk4) inhibitor with IC50 of 136 nM,>10-fold selectivity for closely related kinases Clk1-3 and Dyrk1A/1B. ML167 is found as the first fully selective inhibitor of Clk4 but not other related kinases including Clk1, Clk2 and Clk3 and Dyrk1A/1B. It is 10-fold more potent against Clk4 over other kinases. The treatment of ML167 on Caco-2 cells proves that ML167 is highly cell-permeable. Besides that, ML167 is also found to be an ATP-competitive inhibitor.


Kinase Assay: All compounds were screened using a qHTS approach, where compounds are assayed using at least seven concentrations to generate concentration-response curves for every compounds. The methodology for creating a concentration-titration series between successive copies of library plates for the purpose of large-scale titration-based screening has been described. Briefly, qHTS uses an inter-plate dilution method where the first plate contains the highest concentration of a set of compounds in DMSO, while subsequent plates contain the same compounds in the same well locations, but at successive lower concentrations. Using the protocol outlined above, we calculated a plate throughput of 18 plates/hr, or approximately 7 samples/sec, on the Kalypsys robotic system, which means that a 7 point CRC was obtained every second on the robotic system.


Cell Assay: ML167, as a probe of Clk4, shows high cell permeability in a Caco-2 assay.

In Vivo
Animal model
Formulation & Dosage
ReferencesRosenthal A S, Tanega C, Shen M, et al. An inhibitor of the Cdc2-like kinase 4 (Clk4). 2011.