5-Ethynyluridine 可用于标记新合成的 RNA。这种方法称为使用点击化学 (RICK) 捕获新转录的 RNA 相互作用组，系统地捕获与多种 RNA 结合的蛋白质，包括新生 RNA 和传统上被忽视的非多腺苷酸化 RNA。

5-Ethynyluridine can be used to label newly synthesized RNA. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs.

EU labelling can be used to examine transcription in vivo in mouse models of nervous system disorders. Injection of EU directly into the cerebellum results in reproducible labelling of newly transcribed RNA in cerebellar neurons and glia, with cell type-specific differences in relative labelling intensities, such as Purkinje cells exhibiting the highest levels. EU-labelling accumulating into cytoplasmic inclusions, indicating that EU, like other modified uridines, may introduce non-physiological properties in labelled RNAs[1].

69075-42-9

C11H12N2O6

268.22

PBS (pH 7.2):5 mg/mL
DMF:10 mg/mL
DMSO:10 mg/mL
Powder: -20°C for 3 years
In solvent: -80°C for 2 years