N-Formyl-Met-Leu-Phe 是一种趋化肽和N-甲酰基肽受体的特异性配体，可抑制TNF-alpha的分泌。

N-Formyl-Met-Leu-Phe (fMLF) is a synthetic peptide that acts as an agonist at formyl peptide receptors (FPR; Ki = 0.8 pM).

N-Formyl-Met-Leu-Phe receptor is associated with a Ni GTP regulatory protein, and N-Formyl-Met-Leu-Phe complex is important in the control of several neutrophil functions[1].

N-Formyl-Met-Leu-Phe(fMLP) promoted bone formation in zebrafish and rabbits, suggesting its physiological relevance in vivo[2].

ebrafish (Danio rerio) embryos were collected from spontaneous spawnings reared at 28.5 ℃. Three days postfertilization (～1 day after hatching), feeding of the commercial powder for baby fish was started. Zebrafish embryos were stimulated with fMLP from 8 to 48 h postfertilization (hpf), washed, and raised at 28.5 ℃. At 5 days postfertilization (dpf), 20 zebrafish were stained with Alizarin Red for examination of the effect of fMLP on bone formation. Zebrafish were fixed in 4% paraformaldehyde for 12–18 h. Following fixation, fish were depigmented overnight in 1% KOH containing 3% H2O2 and immersed for 2 h in 0.5% KOH containing 0.05% Alizarin Red, pH 4.2. Alizarin-stained fish were then cleared again in 1% KOH and observed under a microscope[2].

59880-97-6

C21H31N3O5S

437.56

N-Formyl-MLF;酪胺盐酸盐;fMLP

DMSO:82.5 mg/mL (188.55 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years