DMOG 是 α-酮戊二酸辅因子的拮抗剂，是一种 HIF 脯氨酰羟化酶抑制剂，可导致蛋白HIF-1α的积聚和稳定，诱导细胞自噬。它是一种促血管生成剂，在结肠炎和腹泻动物模型中起保护作用。

DMOG, an antagonist of the α-ketoglutarate cofactor, is an inhibitor for HIF prolyl hydroxylase.

DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. [1] DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. [3]

DMOG inhibits endogenous HIF inactivation and induces angiogenesis in the ischaemic skeletal muscles of mice. [2] Up-regulation of hypoxia-inducible factor-1α by DMOG may be the cardioprotective mechanism of ischemic postconditioning in hyperlipidemic rats[4].

To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells. (Only for Reference)

89464-63-1

C6H9NO5

175.14

Dimethyloxalylglycine;Dimethyloxallyl Glycine;Dimethyloxaloylglycine;二甲基草酰甘氨酸

DMSO:17.5 mg/mL(100 mM)
Ethanol:35 mg/mL (199.84 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years