Kuwanon E inhibited cholinesterase enzyme in a dose-dependent manner with K i values ranging between 3.1 and 37.5 μM and between 1.7 and 19.1 μM against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes, respectively. Kuwanon E also inhibited the production of MUC5AC mucin induced by PMA from NCI-H292 cells,.

Nine flavonoids displaying cholinesterase inhibitory activity were isolated from the root bark oiMorus Ihou L., a cultivated edible plant. The isolated compounds were identified as a new flavone (1), 5'-geranyl-5,7,2',4'-tetrahydroxyflavone (2), kuwanon U (3), Kuwanon E (4), morusin (5), morusinol (6), cyclomorusin (7), neocyclomorusin (8), and kuwanon C (9). All compounds apart from compound 6 inhibited cholinesterase enzyme in a dose-dependent manner with K i values ranging between 3.1 and 37.5 μM and between 1.7 and 19.1 μM against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes, respectively. The new compound was char-actierized as S'-geranyl-4'-methoxv-5,7,2'-trihydroxyflavone (1). It showed the most potent inhibitory activity (K i = 3.1 μM for AChE, K i= 1.74 μM for BChE). Lineweaver-Burk and Dixon plots and their secondary replots indicated that flavones (5-9) with prenyl substitution on C-3 were noncompetitive inhibitors, whereas those unsubstituted (1 -4) at C-3 were mixed inhibitors of both AChE and BChE.

68401-05-8

C25H28O6

424.49

5'-[(E)-3,7-二甲基-2,6-辛二烯基]-2',4',5,7-四羟基黄烷酮;桑皮酮 E;桑黄酮 E

(< 1 mg/ml refers to the product slightly soluble or insoluble )
Powder: -20°C for 3 years
In solvent: -80°C for 2 years