PNPP 是用于 ELISA 和常规分光光度测定的碱性和酸性磷酸酶的非蛋白质显色底物。

PNPP is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

Phosphatases catalyze the hydrolysis of PNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP) and the resulting phenolate is yellow, with a maximal absorption at 405 nm[1].

4264-83-9

C6H6NNaO6P

242.08

H2O:160 mg/mL (608.25 mM),Need ultrasonic
Powder: -20°C for 3 years
In solvent: -80°C for 2 years