SY-LB-35 是骨形态发生蛋白 (BMP) 受体的有效激动剂。SY-LB-35 可以刺激 C2C12 成肌细胞系中细胞数量和细胞活力的显著增加，并引起细胞周期向 S 期和 G2/M 期转变。SY-LB-35 刺激典型的Smad和非典型的PI3K/Akt、ERK、p38和JNK胞内信号通路。

SY-LB-35 is a potent agonist of bone morphogenetic protein (BMP) receptor. SY-LB-35 can stimulate significant increases in cell number and cell viability in the C2C12 myoblast cell line, and leads shifts towards the S and G2/M phases of the cell cycle. SY-LB-35 stimulates canonical Smad and non-canonical PI3K/Akt, ERK, p38 and JNK intracellular signaling pathways [1].

SY-LB-35 (0.01-1000 μM; 24 h) stimulates significant increases in cell number and cell viability of C2C12 myoblast cell line [1]. SY-LB-35 (0.01-10 μM; 30 min or 15 min) stimulates Smad phosphorylation and nuclear translocation, activates the PI3K/Akt pathway and direct the cytoplasmic localization of p-Akt, stimulates the phosphorylation and activation of PI3K in the C2C12 cells [1]. SY-LB-35 (0.01-10 μM; 24 h) leads the cell cycle shifting to S and G2/M phases in the C2C12 cells [1]. Cell Viability Assay [1] Cell Line: C2C12 cells Concentration: 0.01, 0.1, 1, 10, 100 and 1000 μM Incubation Time: 24 h Result: Stimulated significant increases in cell number and cell viability. Western Blot Analysis [1] Cell Line: C2C12 cells Concentration: 0.01, 0.1, 1 and 10 μM Incubation Time: 30 or 15 min Result: Stimulated Smad phosphorylation and nuclear translocation, activated the PI3K/Akt pathway and direct the cytoplasmic localization of p-Akt, stimulated the phosphorylation and activation of PI3K in the C2C12 cells. Cell Cycle Analysis [1] Cell Line: C2C12 cells Concentration: 0.01, 0.1, 1 and 10 μM Incubation Time: 24 h Result: Caused the cell cycle shifting to S and G2/M phases.

2603461-70-5

C15H11N3O

249.27

(< 1 mg/ml refers to the product slightly soluble or insoluble )
Powder: -20°C for 3 years
In solvent: -80°C for 2 years