In normal cells, JC-10 selectively aggregated in the mitochondrial matrix to form a reversible red fluorescent polymer (Ex=540 nm, Em=590 nm).In unhealthy mitochondria, due to the decrease or loss of membrane potential, JC-10 is transformed from a polymer into a monomer in the cytoplasm, producing green fluorescence (Ex=490 nm, Em=525 nm).Jc-10 can not only be used for qualitative detection, but also can directly reflect the change of mitochondrial membrane potential due to the change of color.It can also be used for quantitative detection, as the degree of depolarization of mitochondria can be measured by the ratio of red/green fluorescence intensity.The change of these two colors can be detected by the standard filter on flow cytometer. The green fluorescence can be analyzed by FL1 channel, while the red fluorescence can be analyzed by FL2 channel.In addition to flow cytometry, it can also be used in fluorescence imaging and fluorescent plate detection platform.

Prepare a 1X JC-10 working solution: Prepare a 10 to 30 μM 1X working solution in Hanks and 20 mM Hepes buffer (HHBS). Treat cells with test compounds to induce apoptosis. For blank wells, add the corresponding amount of compound buffer. Add 100 μL/well/96-well plate or 25 μL/well/384-well plate of JC-10 working solution into the cell plate. Incubate the plate in a 37 ℃, 5% CO2 incubator for 15-60 min. Monitor the fluorescence change at Ex/Em = 490/525 nm (FITC channel) and 540/590 nm (TRITC channel) for ratio analysis.

TD0056

JC-10

(< 1 mg/ml refers to the product slightly soluble or insoluble )
Powder: -20°C for 3 years
In solvent: -80°C for 2 years