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Triciribine phosphate
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Triciribine phosphate图片
CAS NO:61966-08-3
包装:5mg, 10mg, 50mg
包装与价格:
包装价格(元)
5mg电议
10mg电议
50mg电议

产品名称
NSC-280594, TCN-P
产品介绍

生物活性

Triciribine phosphate (NSC-280594) is a novel potent, small-molecule Akt inhibitor. Triciribine phosphate (NSC-280594) prevents the phosphorylation of Akt. Triciribine phosphate (NSC-280594) disrupts a specific signaling pathway associated with chemoresistance and cancer cell survival in ovarian cancer. Triciribine phosphate (NSC-280594)?in combination with trastuzumab was shown to inhibit cell growth and induce cell death in breast cancer cells that were previously trastuzumab resistant. Triciribine phosphate (NSC-280594) has been demonstrated anti-tumor activity against a wide spectrum of cancers in preclinical and clinical studies. Triciribine phosphate (NSC-280594) is a nucleotide derivative first synthesized by Schram and Townsend.


化学数据

分子量400.28
分子式C13H17N6O7P
CAS号61966-08-3
纯度>98%
溶解性(25°C)DMSO 10 mM
储存和运输条件固体粉末: -20°C 冷藏长期储存
常温运输及临时存放

实验操作 来自于公开的文献,仅供相同实验参考(如实验材料、目的不同,请参考其他文献)

细胞实验
细胞系CEM-SS cells
方法Evaluate Ttriciribine for cytotoxicity by seeding CEM-SS cells at a density of 1 × 104cells/well in growth medium, using a 96-well flat-bottom plate. Serial fivefold dilutions of Triciribine are prepared in growth medium and added to the wells as a second overlay. After a 48-hours incubation at 37 °C, the cells are pulse labeled with [3H]dThd (1 μCi per well, specific activity 20 Ci/mmol) for 6 hours and the cells are harvested to measure total DNA synthesis.
浓度0-500 μM
处理时间48 hours

动物实验
动物模型OVCAR3, OVCAR8, PANC1, OVCAR5 and COLO357 tumor cells are injected s.c. into 80week-old female nude mice.
配制Triciribine is dissolved in 20% DMSO.
剂量1 mg/kg/day
给药处理Triciribine is administrated through i.p. injection once a day.

不同实验动物依据体表面积的等效剂量转换表(数据来源于FDA指南)

小鼠大鼠豚鼠仓鼠
重量 (kg)0.020.151.80.40.0810
体表面积 (m2)0.0070.0250.150.050.020.5
Km系数36128520
动物 A (mg/kg) = 动物 B (mg/kg) × 动物 B的Km系数
动物 A的Km系数

例如,依据体表面积折算法,将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量,需要将20 mg/kg 乘以小鼠的Km系数(3),再除以大鼠的Km系数(6),得到化合物用于大鼠的等效剂量为10 mg/kg。


储备液配制

以下数据基于产品分子量,对于特殊产品,请参照COA中的储备液配制条件和说明进行操作。

Concentration / Solvent Volume / Mass1 mg5 mg10 mg
1 mM2.4983 mL12.4913 mL24.9825 mL
5 mM0.4997 mL2.4983 mL4.9965 mL
10 mM0.2498 mL1.2491 mL2.4983 mL