VX-984 (VX984; M9831; VX 984; M-9831) is a novel, potent and ATP-competitive DNA-PK inhibitor targeting the catalytic subunit of DNA-dependent protein kinase (DNA-PK) with potential antitumor activities. It can sensitize and enhance the activities of both chemo- and radiotherapies. Upon administration, VX-984 binds to and inhibits the catalytic subunit of DNA-PK, thereby interfering with the non-homologous end joining (NHEJ) process and preventing repair of DNA double strand breaks (DSBs) caused by ionizing radiation or chemotherapeutic treatment. This increases chemo- and radiotherapy cytotoxicity and leads to enhanced tumor cell death. The enhanced ability of tumor cells to repair DSBs plays a major role in the resistance of tumor cells to chemo- and radiotherapy; DNA-PK plays a key role in the NHEJ pathway and DSB repair.