| CAS NO: | 2591-17-5 |
| 规格: | ≥98% |
| 包装 | 价格(元) |
| 10mg | 电议 |
| 25mg | 电议 |
| 50mg | 电议 |
| 100mg | 电议 |
| 250mg | 电议 |
| 500mg | 电议 |
| 1g | 电议 |
| Molecular Weight (MW) | 280.32 |
|---|---|
| Formula | C11H8N2O3S2 |
| CAS No. | 2591-17-5 |
| Storage | -20℃ for 3 years in powder form |
| -80℃ for 2 years in solvent | |
| Solubility (In vitro) | DMSO: 56 mg/mL (199.8 mM) |
| Water: <1 mg/mL | |
| Ethanol: <1 mg/mL | |
| Other info | Chemical Name:
(4S)-2-(6-hydroxy-1,3-benzothiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid InChi Key: BJGNCJDXODQBOB-SSDOTTSWSA-N InChi Code: InChI=1S/C11H8N2O3S2/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16/h1-3,7,14H,4H2,(H,15,16)/t7-/m1/s1 SMILES Code: O=C([C@@H]1N=C(C2=NC3=CC=C(O)C=C3S2)SC1)O |
| Synonyms | D-(-)-Luciferin; Firefly luciferin |
| In Vitro | In vitro activity: D-luciferin reacts with luciferase, ATP and oxygen with light emission, and the light is detected by a sensitive photographic film, thereby permitting the visualization of the alkaline phosphatase-conjugated antibodies. |
|---|---|
| In Vivo | In an immunocompetent mouse model of ovarian cancer, use of D-luciferin substrate and firefly luciferase preserves tumor-host immune interactions, since bioluminescent imaging is a more sensitive indication of tumor growth than weight gain. |
| Animal model | Mice |
| Formulation & Dosage | In vivo BLI is performed using a cooled charge-coupled device camera system (IVIS Imaging System 100) 3, 5, 7, 10, 12, 14, 19, 21, 24, and 28 days after the inoculation of HCT116-Luc cells. Mice are injected with 75 mg/kg D-luciferin in 100 μL of phosphate-buffered saline subcutaneously near the scapula and were placed in the light-tight chamber of the imaging system under isoflurane anesthesia. Beginning 5 min after injection, dorsal luminescent images with an exposure time of 1 s are acquired sequentially at a rate of one image per min until 20 min after D-luciferin injection. Data acquisition is continued until 40 min postinjection on days 3 or 5 and until 25 min on day 7, because of the prolonged time course of light emission. Binning is 4 and the field of view is 15 cm. |
| References | J Clin Chem Clin Biochem. 1987 Aug;25(8):511-4; J Immunother Cancer. 2015 May 19;3:16; Int J Biomed Imaging. 2010;2010:471408. |
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