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Coelenterazine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Coelenterazine图片
CAS NO:55779-48-1
规格:≥98%
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)423.46
FormulaC26H21N3O3
CAS No.55779-48-1
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: 84 mg/mL (198.4 mM)
Water: <1 mg/mL
Ethanol: <1 mg/mL
SMILESC1=CC=C(C=C1)CC2=C3N=C(C(=O)N3C=C(N2)C4=CC=C(C=C4)O)CC5=CC=C(C=C5)O
SynonymsOplophorus, luciferin; Coelenterazine; CLZN
实验参考方法
In Vitro

In vitro activity: When monitoring various biological processes, coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine shows a bioluminescence signal. In RIN-5F, 4T1, HCT 116, HEK 293, 293FT, HeLa and INS-1 cells, coelenterazine can be used to assay superoxide anion concentrations


Cell Assay: Coelenterazine showed antioxidative properties in primary cultures of rat hepatocytes subjected to the oxidant tert-butyl hydroperoxide (t-BHP). Coelenterazine (3-10 μM) concentration-dependently increased survival and decreased lipid peroxidation in rat hepatocytes treated for 6 hr with t-BHP (250 μM) [2]. In addition, Coelenterazine (10 μmol/l) can be used to assay superoxide anion concentrations in cancer cell lines.

In Vivothe small molecule coelenterazine (5 mg/kg, intravenous administration) was a potential reporter of cancer-associated superoxide anion in the 4T1 breast adenocarcinoma mouse model [1]. Gli36-Rluc or Gli36-Gluc cells were injected into the leg muscle of nude mice. The mice were injected with 4 mg/kg of coelenterazine and imaged by a cooled charge-coupled device camera.
Animal model5 mg/kg, intravenous administration
Formulation & DosageCoelenterazine (4 μg/g) formulated from an ethanol stock diluted in sodium phosphate buffer (50 mM).
ReferencesMol Ther. 2012 Apr;20(4):692-3. doi: 10.1038/mt.2012.38; Mol Imaging Biol. 2016 Apr;18(2):166-71.