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L-Mimosine(Leucenol)
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
L-Mimosine(Leucenol)图片
CAS NO:500-44-7
规格:≥98%
包装与价格:
包装价格(元)
50mg电议
100mg电议
250mg电议
500mg电议
1g电议
5g电议

产品介绍
理化性质和储存条件
Molecular Weight (MW)198.18
FormulaC8H10N2O4
CAS No.500-44-7
Storage-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)DMSO: <1 mg/mL
Water: <1 mg/mL
10% NaHCO3 (aq): 38 mg/mL (191.74 mM)
Other info

Chemical Name: (S)-alpha-Amino-3-hydroxy-4-oxo-1(4H)-pyridylpropionic acid

InChi Key: RTWFGCUXSRPDOD-QMMMGPOBSA-N

InChi Code: InChI=1S/C8H10N2O4/c9-8(5-11,7(13)14)10-3-1-6(12)2-4-10/h1-4,11H,5,9H2,(H,13,14)/t8-/m0/s1

SMILES Code: O=C(O)[C@@](N)(N(C=C1)C=CC1=O)CO

SynonymsLeucenol; NSC 69188; NSC-69188; NSC69188; (L)-Mimosine
实验参考方法
In Vitro

In vitro activity: L-Mimosine arrests the cell cycle at the G1 phase in PC-3 cells and at the S phase in LNCaP cells, thus attenuating cell proliferation. L-Mimosine, as a prolyl hydroxylase (PHD) inhibitor, stimulates VEGF production, and increases their proangiogenic capacity in dental pulp-derived cells.


Kinase Assay: In rats, it interfered with the reconstitution of the active human prolyl 4-hydroxylase with an IC50 value of 120 μM.


Cell Assay: Cell proliferation in response to L-mimosine is measured using a [3H]thymidine incorporation assay. In this assay, 1 × 104 cells are cultured in each well of a 12-well plate in RPMI-1640 medium with 10% FCS and different concentrations (0–800 μM) of L-mimosine. After the required incubation periods (24 and 48 h), 0.5 μCi/ml of [3H]thymidine is added to each well of the 12-well plate. The cells are then incubated at 37°C in a humidified 5% CO2 atmosphere for 4 h. Cells are then washed twice with cold phosphate-buffered saline (PBS) and then with cold 5% trichloroacetic acid. Cells are lysed by adding 0.5 ml of 0.5 N NaOH. Then 400 μl of the solubilized cell solution are mixed with 4 ml of scintillation cocktail and counted in a liquid scintillation analyzer . Each sample was tested in quadruplicate.

In VivoL-mimosine (50 mg/kg i.p.) substantially upregulates HIF-1alpha expression in the kidneys, and remarkably enhances the natriuretic response to renal perfusion pressure in Sprague-Dawley rats.
Animal modelSprague-Dawley rats
Formulation & DosageDissolved in Na2CO3 solution, pH 8.5; 50 mg/kg; i.p. injection
References

Am J Physiol Cell Physiol. 2012 Feb 15;302(4):C676-85; J Endod. 2012 Nov;38(11):1498-503; Am J Physiol Renal Physiol. 2007 Jan;292(1):F207-16.