| CAS NO: | 500-44-7 |
| 规格: | ≥98% |
| 包装 | 价格(元) |
| 50mg | 电议 |
| 100mg | 电议 |
| 250mg | 电议 |
| 500mg | 电议 |
| 1g | 电议 |
| 5g | 电议 |
| Molecular Weight (MW) | 198.18 |
|---|---|
| Formula | C8H10N2O4 |
| CAS No. | 500-44-7 |
| Storage | -20℃ for 3 years in powder form |
| -80℃ for 2 years in solvent | |
| Solubility (In vitro) | DMSO: <1 mg/mL |
| Water: <1 mg/mL | |
| 10% NaHCO3 (aq): 38 mg/mL (191.74 mM) | |
| Other info | Chemical Name: (S)-alpha-Amino-3-hydroxy-4-oxo-1(4H)-pyridylpropionic acid InChi Key: RTWFGCUXSRPDOD-QMMMGPOBSA-N InChi Code: InChI=1S/C8H10N2O4/c9-8(5-11,7(13)14)10-3-1-6(12)2-4-10/h1-4,11H,5,9H2,(H,13,14)/t8-/m0/s1 SMILES Code: O=C(O)[C@@](N)(N(C=C1)C=CC1=O)CO |
| Synonyms | Leucenol; NSC 69188; NSC-69188; NSC69188; (L)-Mimosine |
| In Vitro | In vitro activity: L-Mimosine arrests the cell cycle at the G1 phase in PC-3 cells and at the S phase in LNCaP cells, thus attenuating cell proliferation. L-Mimosine, as a prolyl hydroxylase (PHD) inhibitor, stimulates VEGF production, and increases their proangiogenic capacity in dental pulp-derived cells. Kinase Assay: In rats, it interfered with the reconstitution of the active human prolyl 4-hydroxylase with an IC50 value of 120 μM. Cell Assay: Cell proliferation in response to L-mimosine is measured using a [3H]thymidine incorporation assay. In this assay, 1 × 104 cells are cultured in each well of a 12-well plate in RPMI-1640 medium with 10% FCS and different concentrations (0–800 μM) of L-mimosine. After the required incubation periods (24 and 48 h), 0.5 μCi/ml of [3H]thymidine is added to each well of the 12-well plate. The cells are then incubated at 37°C in a humidified 5% CO2 atmosphere for 4 h. Cells are then washed twice with cold phosphate-buffered saline (PBS) and then with cold 5% trichloroacetic acid. Cells are lysed by adding 0.5 ml of 0.5 N NaOH. Then 400 μl of the solubilized cell solution are mixed with 4 ml of scintillation cocktail and counted in a liquid scintillation analyzer . Each sample was tested in quadruplicate. |
|---|---|
| In Vivo | L-mimosine (50 mg/kg i.p.) substantially upregulates HIF-1alpha expression in the kidneys, and remarkably enhances the natriuretic response to renal perfusion pressure in Sprague-Dawley rats. |
| Animal model | Sprague-Dawley rats |
| Formulation & Dosage | Dissolved in Na2CO3 solution, pH 8.5; 50 mg/kg; i.p. injection |
| References | Am J Physiol Cell Physiol. 2012 Feb 15;302(4):C676-85; J Endod. 2012 Nov;38(11):1498-503; Am J Physiol Renal Physiol. 2007 Jan;292(1):F207-16. |
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