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NAI-N3
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
NAI-N3图片
CAS NO:1612756-29-2
规格:98%
分子量:228.21
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议

产品介绍
NAI-N3是新型的RNA酰化试剂,用来进行RNA纯化。
CAS:1612756-29-2
分子式:C10H8N6O
分子量:228.21
纯度:98%
存储:Store at -20°C

Background:

NAI-N3 is a novel RNA acylation reagent that enables RNA purification.


The bifunctional NAI-N3 SHAPE reagent is used to label all RNAs inside living cells (or in vitro refolded RNA) to generate covalent modifications on targeted RNAs at single-nucleotide resolution. RNA is isolated, biotinylated using click chemistry and randomly fragmented to a size of 50-100 nt. To generate cDNA sequencing libraries, the RNA fragments are subjected to 3′-end repair, adapter ligation, size selection, biotin enrichment, reverse transcription (RT) and PCR. The end result is selective amplification of fragments corresponding to modified bases in the NAI-N3-treated samples or background expression values from mock-treated samples. Sequencing reads generated from the NAI-N3- and mock (DMSO)-treated samples are mapped to a transcriptome annotation, followed by computational identification of RT stops corresponding to SHAPE modifications—and hence reactive nucleotides—across the entire transcriptome[1].



[1]. Flynn RA, et al. Transcriptome-wide interrogation of RNA secondary structure in living cells with icSHAPE. Nat Protoc. 2016 Feb;11(2):273-90.