CAS NO: | 51805-45-9 |
规格: | 98% |
分子量: | 286.65 |
包装 | 价格(元) |
10g | 电议 |
25g | 电议 |
50g | 电议 |
Reducing Agent
CAS:51805-45-9
分子式:C9H16ClO6P
分子量:286.65
纯度:98%
存储:Store at -20°C
Background:
Tris(2-carboxyethyl)phosphine hydrochloride (TCEP HCL) is a water soluble strong reducing agent that cleave disulfide bonds. It is a non-thiol and non-volatile solid. It can be utizlied as catalyst for the reduction of sulfonyl chlorides, N-oxides, sulfoxides and azides.
In measuring DHA (Dehydroascorbic acid) content in multiple tissues and plasma of 6-weeks-old mice, 35mM TCEP HCL fully reduced DHA to AA (Ascorbic acid) after 2 hours on ice in a 5% solution of metaphosphoric acid containing 1mM ethylenediaminetetraacetic acid (EDTA) at pH 1.5. [1] In Aspergillus saitoi protease type XIII and porcine pepsin, TCEP can also be used in proteolysis and hydrogen/deuterium exchange. [2]
Reference:
1. Sato Y, Uchiki T, Iwama M, Kishimoto Y et al. Determination of dehydroascorbic acid in mouse tissues and plasma by using tris(2-carboxyethyl)phosphine hydrochloride as reductant in metaphosphoric acid/ethylenediaminetetraacetic acid solution. Biol Pharm Bull. 2010;33(3):364-9.
2. Zhang HM, McLoughlin SM, Frausto SD et al. Simultaneous reduction and digestion of proteins with disulfide bonds for hydrogen/deuterium exchange monitored by mass spectrometry. Anal Chem. 2010 Feb 15;82(4):1450-4.