Background:

Fura-2 AM is a ratiometric fluorescent Ca2+ indicator.

The trypsin (100 nM) mediated Ca2+ transients are imagined in tsA-201 cells loaded with either 750 nM (n=111), 500 nM (n=119) or 250 nM (n=130) Fura-2 AM and compare the average Ca2+ increase above the baseline to the value obtained in the initial experiments using 1 μM of Fura-2 AM. The Ca2+ increases above the baseline for the 750, 500 and 250 nM are 633.0±33.9 nM (n=111, P>0.05 compare to 1 μM Fura-2 AM), 552.6±22.7 nM (n=119, P>0.05 compare to 1 μM Fura-2 AM) and 616.0±24.9 nM (n=130, P>0.05 compare to 1 μM Fura-2 AM), respectively[1].

参考文献:
[1]. Tinning PW, et al. A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. J Microsc. 2017 Aug 24.
[2]. Odmara L Barreto-Chang, et al. Calcium Imaging of Cortical Neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23). pii: 1067.