L-Ascorbic acid is essential for the synthesis of collagen
CAS：113170-55-1
分子式：C6H6O9P ? 1.5Mg
分子量：289.5
纯度：98%
存储：Store at 2-8°C

Background:

L-Ascorbic acid is essential for the synthesis of collagen, with deficiency resulting in scurvy.1 Notably, humans and other primates, guinea pigs, and certain other animals lack an enzyme necessary for vitamin C synthesis.[1] L-Ascorbic acid 2-phosphate (AA2P) is a long-acting ascorbic acid derivative that stimulates collagen expression and formation and is used in human cell culture.[2],[3] It may be included in media to enhance the survival of human embryonic stem cells or increase the growth and replicative lifespan of human corneal endothelial cells.[4],[5] AA2P is also used to drive osteogenic differentiation in human adipose stem cells and in human mesenchymal stromal/stem cells.[6],[7],[8]
Reference:

[1]. Gropper, S.S., Smith, J.L., and Groff, J.L. The water-soluble vitamins Advanced Nutrition and Human Metabolism 4, 259-275 (2005).
[2]. Kurata, S., and Hata, R. Epidermal growth factor inhibits transcription of type I collagen genes and production of type I collagen in cultured human skin fibroblasts in the presence and absence of L-ascorbic acid 2-phosphate, a long-acting vitamin C derivative The Journal of Biological Chemisty 266(15), 9997-10003 (1991).
[3]. Yoshikawa, K., Takahashi, S., Imamura, Y., et al. Secretion of non-helical collagenous polypeptides of α1(IV) and α2(IV) chains upon depletion of ascorbate by cultured human cells Journal of Biochemistry 129(6), 929-936 (2001).
[4]. Furue, M.K., Na, J., Jackson, J.P., et al. Heparin promotes the growth of human embryonic stem cells in a defined serum-free medium Proceedings of the National Academy of Sciences of the United States of America 105(36), 13409-13414 (2008).
[5]. Kimoto, M., Shima, N., Yamaguchi, M., et al. Role of hepatocyte growth factor in promoting the growth of human corneal endothelial cells stimulated by L-ascorbic acid 2-phosphate Invest.Ophthalmol.Vis.Sci. 53(12), 7583-7589 (2012).
[6]. Kyll?nen, L., Maimi, S., Mannerstr?m, B., et al. Effects of different serum conditions on osteogenic differentiation of human adipose stem cells in vitro Stem Cell Res.Ther. 4(1), 1-15 (2013).
[7]. Fang, X., Murakami, H., Demura, S., et al. A novel method to apply osteogenic potential of adipose derived stem cells in orthopaedic surgery PLoS One 9(2), 1-6 (2014).
[8]. Mariner, P.D., Johannesen, E., and Anseth, K.S. Manipulation of miRNA activity accelerates osteogenic differentiation of hMSCs in engineered 3D scaffolds J.Tissue Eng.Regen.Med. 6(4), 314-324 (2012).