Na+/Ca2+ exhanger (NCX) inhibitor
CAS：161804-20-2
分子式：C13H14ClN7O.HCl
分子量：356.21
纯度：98%
存储：Store at -20°C

Background:

Benzamil hydrochloride is a specific blocker of sodium channel (ENaC).
The specific ENaC blocker Benzamil hydrochloride adds to the luminal perfusate (10?6 M) significantly decreases baseline [Na+]i by 19.2 mM (n=5) and almost completely inhibits the [NaCl]L-dependent increasing in [Na+]i when coadministers with luminal AngII[2].
Intracerebroventricular injection of endothelin-1 (1 nmol) after intracerebroventricular preinjection of the vehicle of Benzamil hydrochloride markedly increases both mean arterial pressure and abdominal sympathetic nervous activity for 10 min. In SHRSP, intracerebroventricular infusion either of 1 or 10 nmol/kg/day Benzamil hydrochloride decreases systolic blood pressure (4th day: F=6.131, 1 nmol/kg/day, P<0.05, 10 nmol/kg/day, P<0.01; 5th day: F=6.842, 1 nmol/kg/day, P<0.05, 10 nmol/kg/day, P<0.01; 6th day: F=5.988, 1 nmol/kg/day, P<0.05, 10 nmol/kg/day, P<0.01; 7th day: F=7.935, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P<0.01), urinary excretion of vasopressin (5th day: F=9.385, 1 nmol/kg/day, P<0.05, 10 nmol/kg/day, P<0.05; 6th day: F=8.783, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P<0.01; 7th day: F=8.996, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P< 0.01), and norepinephrine (3rd day: F=4.341, 1 nmol/kg/day, P<0.05, 10 nmol/kg/day, P<0.01; 4th day: F=9.865, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P<0.01; 5th day: F=14.652, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P<0.01; 6th day: F=13.376, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P<0.01; 7th day: F=8.594, 1 nmol/kg/day, P<0.01, 10 nmol/kg/day, P<0.01) compare with vehicle[1].
Reference：
[1]. Nishimura M, et al. Benzamil blockade of brain Na+ channels averts Na(+)-induced hypertension in rats. Am J Physiol. 1998 Mar;274(3 Pt 2):R635-44.
[2]. Peti-Peterdi J, et al. Angiotensin II directly stimulates ENaC activity in the cortical collecting duct via AT(1) receptors. J Am Soc Nephrol. 2002 May;13(5):1131-5.