Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).
CAS：70217-82-2
分子式：C26H21N3O
分子量：391.5
纯度：98%
存储：Store at -20°C

Background:

Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc). It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h , which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols. Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10. BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.