Background:

DiD perchlorate is a lipophilic cyanine dye.

Two weeks after injection of stained cells, a single, bright, DiD perchlorate (DiD)-positive cells located between 15 to 40 µm from the endosteum is found. Results reveal a progressive appearance of cell clusters of decreased dye intensity, consistent with the partitioning of DiD perchlorate label on cell division[1].

[1]. Lo Celso C, et al. Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche.