L-Arginine serves as a common substrate for both nitric oxide synthase (NOS) and arginase in the cell.
货号:ajcx19020
CAS:N/A
分子式:C6H13N3O2S•HBr
分子量：272.2
溶解度:PBS (pH 7.2): 3 mg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

L-Arginine serves as a common substrate for both nitric oxide synthase (NOS) and arginase in the cell. NOS catalyzes the oxidation of arginine to citrulline and NO with Nω-hydroxy-L-arginine (NOHA) formed as an intermediate. Arginase, on the other hand, catalyzes the hydrolysis of arginine into urea and L-ornithine. Thioarginine (hydrobromide) is a colorimetric substrate for arginase that provides the basis for continuous spectrophotometric measurement of enzyme activity. It exhibits Km and kcat values of 0.5 mM and 18,000 min-1, respectively, which are similar to physiological substrate arginine. The thiol product of the reaction reacts with DTNB to produce 2-nitro-5-thiobenzoate for easy visualization at 405-420 nm.