306Oi10 是一种支链可电离类脂质，可用于构建脂质纳米颗粒 (LNP) 以传递信使 RNA。
货号:ajcx28360
CAS:2322290-93-5
分子式:C59H115N3O8
分子量：994.6
溶解度:DMF: 20 mg/ml,DMSO: 33 mg/ml,Ethanol: 33 mg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

306O_i10is a branched-chain ionizable lipidoid.¹It has been used in the generation of lipid nanoparticles (LNPs). It has strong surface ionization at pH 5, which is the pH in the late endosomal compartment, and a surface pK_avalue of 6.4 when encapsulating mRNA. LNPs containing 306O_i10and encapsulating an mRNA reporter accumulate primarily in the mouse liver and those encapsulating erythropoietin increase serum erythropoietin levels in mice. LNPs containing 306O_i10and encapsulating mRNA encoding the Cas9 nuclease (mCas9) and single-guide RNA targeting LoxP (sgLoxP) have been used to induce CRISPR-mediated gene editing in Ai9 mice that express a floxed STOP codon to prevent tdTomato expression.²

1.Hajj, K.A., Ball, R.L., Deluty, S.B., et al.Branched-tail lipid nanoparticles potently deliver mRNA in vivo due to enhanced ionization at endosomal pHSmall15(6)e1805097(2019) 2.Hajj, K.A., Melamed, J.R., Chaudhary, N., et al.A potent branched-tail lipid nanoparticle enables multiplexed mRNA delivery and gene editing in vivoNano Lett.20(7)5167-5175(2020)