An internal standard for the quantification of cyclic di-GMP
货号:ajcx26740
CAS:N/A
分子式:[13C]20H22[15N]10O14P2 • 2Na
分子量：764.2
溶解度:Water: soluble
纯度：98%
存储：Store at -20°C
库存：现货

Background:

¹³C₂₀,¹⁵N₁₀-Cyclic di-GMP is intended for use as an internal standard for the quantification of cyclic di-GMP by GC- or LC-MS. Cyclic di-GMP is a second messenger in bacteria involved in diverse prokaryotic processes, including biofilm formation, motility, virulence, and cell cycling.^1,2In eukaryotic cells, cyclic di-GMP is detected by and binds to the transmembrane protein stimulator of interferon genes (STING; K_d= 1.21 µM), leading to activation of the innate immune system.^3,4It has been used at a preset molar ratio with STING dimers in binding assays to determine the binding constants of particularly tight binding partners, such as 2’3’-cGAMP. Cyclic di-GMP induces IFN-β mRNA expressionin vitro(EC₅₀= 537.8 nM) but less potently than 2’3’-cGAMP , 3’2’-cGAMP, 3’3’-cGAMP , and 2’2’-cGAMP .

1.RÖmling, U., Galperin, M.Y., and Gomelsky, M.Cyclic di-GMP: The first 25 years of a universal bacterial second messengerMicrobiol. Mol. Biol. Rev.77(1)1-52(2013) 2.MartÍnez, L.C., and Vadyvaloo, V.Mechanisms of post-transcriptional gene regulation in bacterial biofilmsFront. Cell. Neurosci.4(38)1-15(2014) 3.Schaap, P.Cyclic di-nucleotide signaling enters the eukaryote domainIUBMB Life65(11)897-903(2013) 4.Zhang, X., Shi, H., Wu, J., et al.Cyclic GMP-AMP containing mixed phosphodiester linkages is an endogenous high-affinity ligand for STINGMol. Cell51(2)226-235(2015)