Alkynetyramide是一种抗坏血酸过氧化物酶2(APEX2)探针。Alkynetyramide显著提高了完整酵母细胞的APEX标记效率，比APEX2底物生物素苯酚(BP)具有更大的细胞壁渗透性。Alkynetyramide还有助于鉴定APEX标记位点，明确线粒体蛋白的膜拓扑的分配。
货号:ajcx30142
CAS:1694495-59-4
分子式:C13H15NO2
分子量：217.26
溶解度:DMSO: 100 mg/mL (460.28 mM)
纯度：98%
存储：Store at -20°C
库存：现货

Background:

Alkyne tyramide is a clickable ascorbate peroxidase 2 (APEX2) probe. Alkyne tyramide substantially improves APEX-labeling efficiency in intact yeast cells, as it is more cell wall-permeant than APEX2 substrate biotin-phenol (BP). Alkyne tyramide also facilitates the identification of APEX-labeling sites, allowing the unambiguous assignment of membrane topology of mitochondrial proteins[1].

Yeast cells expressing Su9-APEX2 are incubated with each probe at 2.5 mM final concentration for half an hour, and the labeling reaction is initiated by the addition of 1 mM H2O2. Alkyne tyramide is the most reactive APEX2 substrate for labeling the yeast proteome[1].APEX2-mediated Alkyne tyramide labeling in the yeast mitochondria is more effective at probe concentrations above 1 mM[1].Alkyne tyramide enables proteomic profiling in the mitochondrial matrix of intact yeast cells with exceptionally high specificity (94%), and offers higher coverage than the traditional APEX2 substrate biotin-phenol (BP)[1].

[1]. Li Y, et al. A Clickable APEX Probe for Proximity-Dependent Proteomic Profiling in Yeast. Cell Chem Biol. 2020;27(7):858-865.e8.