MY-875

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MY-875

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

包装与价格：

包装	价格(元)
5mg	电议
10mg	电议
25mg	电议
50mg	电议
100mg	电议

产品介绍

MY-875 是一种竞争性的微管蛋白聚合 (microtubulin polymerization) 抑制剂, IC50 值为 0.92 μM。MY-875 通过靶向秋水仙碱结合位点抑制微管蛋白聚合，同时可以激活 Hippo 通路。MY-875 可以诱导细胞凋亡 (apoptosis)，具有抗癌活性。

分子式

C₂₁H₂₅NO₆

分子量

387.43

溶解度

DMSO : ≥ 250 mg/mL (645.28 mM)

储存条件

4°C, protect from light

General tips

For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.

Shipping Condition

Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

产品描述

MY-875 is a competitivemicrotubulin polymerizationinhibitor with anIC₅₀value of 0.92 μM. MY-875 inhibits microtubulin polymerization by targeting colchicine binding sites and activates the Hippo pathway. MY-875 inducesapoptosisand has anticancer activity^[1].

MY-875 (0-80 μM, 48 h) has significant anti-proliferative activity against cancer cells^[1].
MY-875 (1-10 μM) can inhibit microtubule protein polymerization with an IC₅₀value of 0.92 μM while inhibiting alkylation of β-tubulin and the formation of EBI-β-tubulin adduct bands in a dose-dependent manner^[1].
MY-875 (0-45 nM, 48 h) can induce the phosphorylation state of MST (Ste20-like kinases) and LATS (large tumor suppressor kinases), leading to YAP (Yes-associated protein) degradation in a dose-dependent manner^[1].
MY-875 (0-45 nM, 24 h) significantly inhibits cell colony-forming ability, arrests cells in the G2/M phase and induces cell apoptosis in a dose-dependent manner^[1].

Cell Proliferation Assay^[1]

Cell Line:	MGC-803, HCT-116, KYSE450, HGC-27, SGC-7901cell lines
Concentration:	0-80 μM
Incubation Time:	48 hours
Result:	Inhibited the proliferation of MGC-803, HCT-116, KYSE450, HGC-27 and SGC-7901 cells with the IC₅₀values of 0.027, 0.055, 0.067, 0.033 and 0.025 μM, respectively. Showed strong inhibitory effect on other tumor cell lines with the IC₅₀values less than 0.1 μM, such as DU145, A549, MCF-7, etc.

Cell Cycle Analysis^[1]

Cell Line:	MGC-803, SGC-7901 cell lines
Concentration:	0-45 nM
Incubation Time:	24 hours
Result:	Increased the percentage of cells in G2/M phase from 19.38% to 76.97% in MGC-803 cells and from 7.04% to 80.89% in SGC-7901 cells, respectively at 45 nM.

Apoptosis Analysis^[1]

Cell Line:	MGC-803, SGC-7901 cell lines
Concentration:	0-45 nM
Incubation Time:	48 hours
Result:	Induced apoptotic cells from 21.96% to 76.08% in MGC-803 cells and from 9.28% to 63.51% in SGC-7901 cells, respectively at 45 nM. Reduced expression of anti-apoptotic proteins c-IAP1, Bcl-xL and Mcl-1.