Cell lines

Human red blood cells (RBCs)

Preparation method

The solubility of this peptide in sterile water is >10 mM. Stock solution should be splited and stored at -80℃ for several months.

Reaction Conditions

200 μM,

Applications

10Panx reduced ATP release in response to lowered pO2 by 90.9±15.5%. It did not reduce ILO-induced ATP release.

Animal models

Dosage form

Applications

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

10Panx is a mimetic inhibitory peptide of panx1, which is a mammalian protein ectopically expressed to function as a hemi-channel, that readily and reversibly inhibits the panx1 currents. 10Panx has attracted increasing attention as it allows scientists readily and reliably to examine inhibition of panx1 in cells where efficient and consistent delivery of siRNA is highly difficult. According to results of multiple previous studies, 10Panx selectively inhibits P2X₇R-induced dye uptake without altering other aspects of R2X₇R activation and ATP-evoked membrane currents, constitutive dye-uptake and hemichannel-like currents that are induced by the overexpression of panx1, and ATP-mediated IL-1β release.

Reference

[1].Pablo Pelegrin and Annmarie Surprenant. Pannexin-1 mediates large pore formation and interleukin-1β release by the ATP-gated P2X₇receptor. The EMBO Journal (2006) 25, 5071-5082