包装 | 价格(元) |
1mg | 电议 |
5mg | 电议 |
10mg | 电议 |
25mg | 电议 |
Brain natriuretic peptide
Cell lines | Normal adult canine ventricular fibroblasts |
Preparation method | The solubility of this peptide in sterile water is >10 mM. Stock solution should be splited and stored at -80℃ for several months. |
Reaction Conditions | 1 μM, 5 min |
Applications | BNP (1 μM) significantly increased the intracellular cGMP, whereas lower concentrations did not alter the cGMP. At this concentration, BNP elevated cGMP levels with a maximal effect at 5 minutes. BNP (1 μM) significantly inhibited the [3H]proline incorporation into the cells by 29%. |
Animal models | Adult Japanese white rabbits |
Dosage form | Injected through the sclera into the vitreous cavity, 100 μM, 4 hours |
Applications | At 100 μM, BNP treatment induced a significant decrease in IOP compared with vehicle-treated eyes. In particular, there were statistically significant differences at 4 and 6 hours. In addition, BNP treatment at 10 μM caused a significant decrease in IOP compared to the vehicle-treated eyes, but only at 6 hours after the injection. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
产品描述 | Basic natriuretic peptide (BNP), now known as B-type natriuretic peptide (also BNP) or GC-B, is a 32 amino acid polypeptide secreted by the ventricles of the heart in response to excessive stretching of heart muscle cells (cardiomyocytes). The release of BNP is modulated by calcium ions1. BNP is named as such because it was originally identified in extracts of porcine brain, although in humans it is produced mainly in the cardiac ventricles. The physiologic actions of BNP are similar to ANP and include decrease in systemic vascular resistance and central venous pressure as well as an increase in natriuresis. Thus, the net effect of BNP and ANP is a decrease in blood volume which lowers systemic blood pressure and afterload, yielding an increase in cardiac output, partly due to a higher ejection fraction. The main clinical utility of either BNP or NT-BNP is that a normal level rules out acute heart failure in the emergency setting2. Recombinant BNP, Nesiritide is used to treat decompensated heart failure3. References: |